2011 Fiscal Year Final Research Report
The development of novel molecular target drug : Identification of RNA aptamer targeting human epidermal growth factor by SELEX
| Project/Area Number |
22700918
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Clinical oncology
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| Research Institution | Hiroshima University |
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Principal Investigator |
HAMAI Yoichi 広島大学, 病院, 病院助教 (90423384)
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| Research Collaborator |
HIHARA Jun 広島大学, 原爆放射線医科学研究所, 講師 (10322744)
EMI Manabu 広島大学, 原爆放射線医科学研究所, 助教 (30464308)
AOKI Yoshiro 広島大学, 病院, 医科診療医 (70557074)
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| Project Period (FY) |
2010 – 2011
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| Keywords | SELEX法 / RNAアプタマー / EGF / 分子標的薬 |
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| Research Abstract |
The biotin tagged epidermal growth factor (EGF) protein was made. The specific RNA aptamer for EGF protein was identified and acquired from synthetic oligonucleotide with random-sequence by systematic evolution of ligands by exponential enrichment (SELEX) method using the biotin tagged EGF protein. This anti-EGF aptamer could interfere with the binding of EGF to the receptor.
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