Development research on immuno-gene therapy for malignant B-cell lymphoma

2011 Fiscal Year Final Research Report

• Project/Area Number: 22700923
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Clinical oncology
• Research Institution: Jichi Medical University
• Principal Investigator: TSUKAHARA Tomonori 自治医科大学, 医学部, 助教 (10362120)
• Project Period (FY): 2010 – 2011
• Keywords: 悪性リンパ腫 / T細胞 / 腫瘍標的化 / 遺伝子治療

Research Abstract

For the treatment of refractory malignant B-cell lymphoma(B-cell non-Hodgkin lymphoma), we conducted experiments to develop an adoptive immuno-gene therapy using T-cells expressing a CAR(chimeric antigen receptor) targeting CD19.We demonstrated that genetically engineered T-cells efficiently lyzed CD19-positive B-cell lymphoma in vitro and in a mouse xenograft model. These results indicate that functional engineered T-cells would be effective for the treatment of refractory B-cell non-Hodgkin lymphoma.

Research Products

• [Journal Article] Interleukin-10 expression induced by adeno-associated virus vector suppresses proteinuria in Zucker obese rats (2011)
  • Author(s): Ogura M, Urabe M, Akimoto T, Onishi A, Ito C, Ito T, Tsukahara T, Mizukami H, Kume A, Muto S, Kusano E, and Ozawa K
  • Journal Title: Gene Ther
  • DOI: doi:10.1038/gt.2011.183.
  • Peer Reviewed
• [Journal Article] キメラ抗原受容体発現T細胞を用いた難治性B細胞腫瘍に対する養子免疫遺伝子療法 (2011)
  • Author(s): 塚原智典
  • Journal Title: 医学のあゆみ Volume: 237 Pages: 223-226
• [Journal Article] キメラ抗原受容体発現T細胞による癌に対する養子免疫遺伝子療法 (2010)
  • Author(s): 塚原智典
  • Journal Title: 最新医学 Volume: 65 Pages: 1813-1818
• [Presentation] Engineered T lymphocytes expressing an anti-CD19 CAR specifically eradicate B-lymphoma cells (2011)
  • Author(s): Tsukahara, T., Ohmine, K., Uchibori, R., Urabe, M., Mizukami, H., Kume, A., Riviere, I., Sadelain, M., Brentjens, R. J., Ozawa K.
  • Organizer: Annual Meeting of Japan Society of Gene Therapy
  • Place of Presentation: Fukuoka
  • Year and Date: 2011-07-07
• [Presentation] Validation of anti-tumor effects mediated by anti-CD19CAR T-cells for B cell lymphoma (2010)
  • Author(s): Tsukahara, T., Ohmine, K., Uchibori, R., Urabe, M., Mizukami, H., Kume, A., Ozawa, K.
  • Organizer: XXIII Annual Congress of the European Society of Gene and Cell Therapy
  • Place of Presentation: Milano, Italy
  • Year and Date: 2010-10-23
• [Presentation] Cytotoxicity of primary T-lymphocytes expressing a chimeric antigen receptor targeting CD19 against B-cell lymphoma cells (2010)
  • Author(s): Tsukahara, T., Ohmine, K., Uchibori, R., Urabe, M., Mizukami, H., Kume, A., Ozawa, K.
  • Organizer: Annual Meeting of the Japanese Cancer Association
  • Place of Presentation: Osaka
  • Year and Date: 2010-09-22
• [Presentation] Bリンパ腫細胞に対する抗CD19キメラ抗原受容体発現T細胞の細胞障害活性 (2010)
  • Author(s): 塚原智典, 大嶺謙, 内堀亮介, 卜部匡司, 水上浩明, 久米晃啓, 小澤敬也
  • Organizer: 造血器腫瘍免疫療法研究会
  • Place of Presentation: 松山
  • Year and Date: 2010-08-29
• [Presentation] Ex vivo expansion of primary T-lymphocytes expressing a chimeric antigen receptor targeting CD19 with antigen stimulation (2010)
  • Author(s): Tsukahara, T., Sakurai, C., Ohmine, K., Uchibori, R., Urabe, M., Mizukami, H., Kume, A., Riviere, I., Sadelain, M., Brentjens, R. J., Ozawa K.
  • Organizer: Annual Meeting of Japan Society of Gene Therapy
  • Place of Presentation: Utsunomiya
  • Year and Date: 2010-07-01