Analysis of a new mechanism on homology-dependent repair

2011 Fiscal Year Final Research Report

• Project/Area Number: 22710053
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Risk sciences of radiation/Chemicals
• Research Institution: Kyoto University
• Principal Investigator: KATO Akihiro 京都大学, 放射線生物研究センター, 研究員 (70423051)
• Project Period (FY): 2010 – 2011
• Keywords: DNA修復 / 相同組換え

Research Abstract

In order to clarify the biological significance of the physical interaction between NBS1 and RAD51, we tried to establish a NBS1 mutant that was not able to bind to RAD51. In this process, we found that NBS1 interacted with RAD51 through MRE11. The MRE11 mutant that could not interact with RAD51 had full activity of homology-dependent repair of DNA double-strand breaks(DSBs). Cells expressing the MRE11 mutant did not show sensitivity to ionizing radiation. These results indicate that the interaction between NBS1 and RAD51 does not have an important role in DSB repair but has a role in other mechanisms.

Research Products

• [Journal Article] Genistein, isoflavonoids in soybeans, prevents the formation of excess radiation-induced centrosomes via p21up-regulation (2011)
  • Author(s): Shimada M, Kato A, Habu T, Komatsu K
  • Journal Title: Mutatation Research Volume: Volume 716 Pages: 27-32
  • DOI: DOI:10.1016/j.mrfmmm.2011.07.017
  • Peer Reviewed
• [Journal Article] HERC2 Interacts with Claspin and regulates DNA origin firingandr eplication fork progression (2011)
  • Author(s): Izawa N, Wu W, Sato K, Nishikawa H, Kato A, Boku N, Itoh F, Ohta T
  • Journal Title: Cancer Research Volume: Volume 71 Pages: 5621-5625
  • DOI: DOI:10.1158/0008-5472.CAN-11-0385
  • Peer Reviewed
• [Journal Article] NBS1recruits RAD18 viaa RAD6-like do mainand regulates Polη-dependent translesion DNA synthesis (2011)
  • Author(s): Yanagihara H, Kobayashi J, Tateishi S, Kato A, Matsuura S, Tauchi H, Yamada K, Takezawa J, Sugasawa K, Masutani C, Hanaoka F, Weemaes CM, Mori T, Zou L, Komatsu K
  • Journal Title: Molecular Cell Volume: Volume 43 Pages: 788-797
  • DOI: DOI:10.1016/j.molcel.2011.07.026
  • Peer Reviewed
• [Journal Article] Regulation of homologous recombination by RNF20-dependent H2B ubiquitination (2011)
  • Author(s): Nakamura K, Kato A, Kobayashi J, Yanagihara H, Sakamoto S, Oliveira DV, Shimada M, Tauchi H, Suzuki H, Tashiro S, Zou L, Komatsu K.
  • Journal Title: Molecular Cell Volume: Volume 43 Pages: 515-528
  • DOI: DOI:10.1016/j.molcel.2011.02.002
  • Peer Reviewed
• [Presentation] MRN複合体とRAD51の相互作用 (2011)
  • Author(s): 加藤晃弘
  • Organizer: 日本放射線影響学会第54回大会
  • Place of Presentation: 神戸
  • Year and Date: 2011-11-18
• [Presentation] Physical and functional interaction between MRN complex and RAD51 (2011)
  • Author(s): 加藤晃弘
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋
  • Year and Date: 2011-10-05
• [Presentation] The link between MRN complex and RAD51 (2011)
  • Author(s): 加藤晃弘
  • Organizer: ICRR2011
  • Place of Presentation: ポーランド
  • Year and Date: 2011-08-30
• [Presentation] The link between MRN complex and RAD51 (2011)
  • Author(s): 加藤晃弘
  • Organizer: ICRR2011
  • Place of Presentation: 京都
  • Year and Date: 2011-08-30
• [Presentation] MRN複合体とRAD51との関係 (2010)
  • Author(s): 加藤晃弘
  • Organizer: 日本放射線影響学会第53回大会
  • Place of Presentation: 大阪
  • Year and Date: 2010-10-20
• [Presentation] Control of homologous recombination by RNF20-dependent H2B ubiquitination (2010)
  • Author(s): 加藤晃弘
  • Organizer: EMBO workshop「The Interface between the Ubiquitin Family and the DNA Damage Response」
  • Place of Presentation: クロアチア
  • Year and Date: 2010-09-04