Design and Preparation of a Nanodevice Containing Gold Nanoparticles for Delivery and Imaging of Nucleic Acids

2011 Fiscal Year Final Research Report

• Project/Area Number: 22710114
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Nanomaterials/Nanobioscience
• Research Institution: University of Tsukuba
• Principal Investigator: OISHI Motoi 筑波大学, 数理物質系, 講師 (90419242)
• Project Period (FY): 2010 – 2011
• Keywords: 金ナノ粒子 / ナノデバイス / ナノゲル粒子 / siRNA

Research Abstract

A PEGylated nanogel containing gold nanoparticles and siRNAs (nanodevice) was designed and prepared for delivery of nucleic acids and imaging of cancer. The nanodevice shows efficient release of siRNAs in response to intracellular environments, because the thiol-Au interaction caused an exchange reaction between siRNA-SH/Au and thiol-containing glutathione molecules, allowing the efficient RNAi activity in cancer cells. Additionally, the nanodevice also serves as an X-ray CT contrast agent due to the large payload of gold nanoparticles in the core of nanogel. The PEGylated nanogel described here is suitable as a smart nanodevice for delivery of nucleic acids and imaging of cancer.

Research Products

• [Journal Article] Dependence of Swelling Behavior of pH-responsive PEG-modified Nanogel on Cross-link Density (2012)
  • Author(s): Y. Shinohara, G. Tamura, A. Tamura, Y. Sanada, M. Oishi, I. Akiba, Y. Nagasaki, K. Sakurai and Y Amemiya
  • Journal Title: Polym. J Volume: 44 Pages: 240-244
  • DOI: DOI:10.1038/pj.2011.123
  • Peer Reviewed
• [Journal Article] Systemic Delivery of siRNA to Tumors Using a Lipid Nanoparticle Containing a Tumor Specific Cleavable PEG-lipid (2011)
  • Author(s): H. Hatakeyama, H. Akita, E. Itho, Y. Hayashi, M. Oishi, Y. Nagasaki, R. Danev, K. Nagayama, N. Kaji, H. Kikuchi, Y. Baba and H. Harashima
  • Journal Title: Biomaterials Volume: 32 Pages: 4306-4316
  • DOI: DOI:10.1016/j.biomaterials.2011.02.045
  • Peer Reviewed
• [Journal Article] Stimuli-Responsive Smart Nanogels for Cancer Diagnostics and Therapy (2010)
  • Author(s): M. Oishi and Y. Nagasaki
  • Journal Title: Nanomedicine Volume: 5 Pages: 451-468
  • DOI: DOI:10.2217/nnm.10.18
  • Peer Reviewed
• [Presentation] Design of PEGylated Polyamine Gold Nanoparticles as Nucleic Acid Drug Carriers for Systemic Cancer Gene Therapy (2011)
  • Author(s): M. Oishi, et.al
  • Organizer: 第60回高分子学会年次会
  • Place of Presentation: 大阪国際会議場(大阪)
  • Year and Date: 2011-05-26
• [Presentation] Boron Neutron Capture Therapy Assisted by Nanoparticles : Enhanced Tumor Accumulation by Core-Polymerization with Boron-Containing Monomer (2011)
  • Author(s): M. Oishi, et. al
  • Organizer: 第60回高分子学会年次会
  • Place of Presentation: 大阪国際会議場(大阪)
  • Year and Date: 2011-05-26
• [Presentation] ピンポイント治療および診断を目指した刺激応答性PEG化ナノゲル粒子の創製 (2010)
  • Author(s): 大石基
  • Organizer: 第59回高分子学会年次会
  • Place of Presentation: パシフィコ横浜(神奈川)
  • Year and Date: 2010-05-26
• [Book] Stimuli-Responsive PEGylated Nanogels for Smart Nanomedicines Chapter 5 in the book entitled Biomedical Applications of Hydrogels Handbook (2010)
  • Author(s): M. Oishi and Y. Nagasaki
  • Total Pages: 87-105
  • Publisher: Springer Inc