2011 Fiscal Year Final Research Report
High-throughput definition of transcriptional units to grasp global expression states of non-coding RNA in budding yeast cells
| Project/Area Number |
22710182
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Genome biology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
MIURA Fumihito 東京大学, 大学院・理学系研究科, 特任助教 (50447348)
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| Project Period (FY) |
2010 – 2011
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| Keywords | トランスクリプトーム |
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| Research Abstract |
Simultaneous determinations of 5' and 3' terminal sequences of a mRNA molecule were conducted on budding yeast Saccharomyces cerevisiae with highly parallelized manner. Logarithmically growing cells, and cells before and six times every 2 hours after induction of sporulation were collected. Cloning-free GIS were applied for then, and sequencing was performed on a next-generation sequencer. Construction of a unique database storing transcription initiation and termination position of each budding yeast transcript as a pair is currently underway.
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