2012 Fiscal Year Final Research Report

Functional analysis of chromatin dynamics during aging by genomic approach

Research Project

Project/Area Number	22710183
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Genome biology
Research Institution	Niigata University (2011-2012) Tokyo Institute of Technology (2010)
Principal Investigator	SAKAI Akiko 新潟大学, 医歯学総合研究科, 特任助教 (70532745)
Research Collaborator	KUWANO Ryozo 新潟大学, 脳研究所, 教授 (20111734) SHIRAHIGE Katsuhiko 東京大学, 分子細胞生物学研究所, 教授 (90273854) NAKATO Ryuichiro 東京大学, 分子細胞生物学研究所, 助教 (60583044)
Project Period (FY)	2010 – 2012
Keywords	ゲノム / ChIP-seq / 転写制御 / クロマチン
Research Abstract	Chromatin structure has fundamental roles in gene expression, which could bere-organized as conditions and circumstances of the cells change. In this study, wehave tried to understand the molecular mechanism and the roles of the structuralchanges in chromatin during aging. ChIP-seq method was applied to identify thebinding sites of chromatin factors throughout the genome in mammalian brain. Ourresults indicated that during the critical period in which the plasticity of the brainincreases, Otx2, the essential transcription factor, and cohesin, the regulator of higherorder chromatin structure, act cooperatively for gene regulation.

(3 results)