2011 Fiscal Year Final Research Report
Comprehensive analysis of miRNA promoters
| Project/Area Number |
22710189
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Genome biology
|
|---|
| Research Institution | The Institute of Physical and Chemical Research |
|---|
Principal Investigator |
KAWAJI Hideya 独立行政法人理化学研究所, 小分子RNA連携ユニット, ユニットリーダー (20525406)
|
|---|
| Project Period (FY) |
2010 – 2011
|
| Keywords | miRNA / primary transcript / promoter / transcriptome / transcriptional regulation / small RNA / next generation sequencer |
|---|
| Research Abstract |
We performed siRNA knockdown experiments against factors contributing the miRNA biogenesis, and profiled nuclear RNA with high-throughput sequencing technologies. With HeliScopeCAGE that profile TSS activities quantitatively, we identified 24 promoters on sixty miRNAs. With RNA-seq, we identified more than half of the 24 miRNA promoters.
|
