2011 Fiscal Year Final Research Report
RNAi screening for genes involved in differentiation of embryonic stem cells into primordial germ cells.
| Project/Area Number |
22710197
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
System genome science
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| Research Institution | Tohoku University |
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Principal Investigator |
MAEDA Ikuma 東北大学, 加齢医学研究所, 助教 (20376560)
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| Project Period (FY) |
2010 – 2011
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| Keywords | 発生分化 |
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| Research Abstract |
We identified Max as a repressor of germ-cell-related genes in embryonic stem cells (ESCs)via RNAi screen. Transcriptome analysis revealed that Max knockdown (KD)in ESCs resulted in selective, global derepression of germ-cell-specific genes. Max interacted with histone H3K9 methyltransferases and associated with the promoter regions of germ-cell-specific genes in ESCs. In addition, Max-KD resulted in a decrease in histone H3K9 dimethylation at those promoter regions.
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