Isolation and characterization of prenyltransferases in furanocoumarin biosynthesis

2011 Fiscal Year Final Research Report

• Project/Area Number: 22710214
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Living organism molecular science
• Research Institution: Kyoto University
• Principal Investigator: KOEDUKA Takao 京都大学, 化学研究所, 助教 (30565106)
• Project Period (FY): 2010 – 2011
• Keywords: クマリン / プレニル基転移酵素 / 生合成

Research Abstract

In this study, we characterized the biosynthetic gene encoding to the coumarin-specific prenyltransferases to understand the molecular mechanism underlying the biosynthesis of multifunctional and bioactive prenylated coumarins that are produced in plants. We have detected both O-and C-prenyltransferase activities for coumarin substrates in the microsome fraction prepared from lemon(Citrus limon) peel, where large amounts of prenylated coumarins are accumulated. Moreover, we succeeded to isolate the full length of cDNAs, which would be encoding to coumarin-specific prenyltransferases, four genes from Rutaceae and two genes from Apiaceae.

Research Products

• [Journal Article] Characterization of Coumarin-specific Prenyltransferase Activities in Citrus limon Peel
  • Author(s): Munakata, R., Inoue, T., Koeduka, T., Sasaki, K., Tsurumaru, Y., Sugiyama, A., Uto, Y., Hori, H., Azuma, J., Yazaki, K.
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: (印刷中)
  • Peer Reviewed
• [Presentation] レモン果皮におけるクマリン基質プレニルトランスフェラーゼの検出とcDNAクローニング (2012)
  • Author(s): 棟方涼介、井上剛史、肥塚崇男、佐々木佳菜子、鶴丸優介、杉山暁史、宇都義浩、堀均、東順一、矢崎一史
  • Organizer: 日本農芸化学会2012年度大会
  • Place of Presentation: (京都市)京都女子大学
  • Year and Date: 2012-03-23
• [Presentation] オイゲノール及び関連物質の抗菌活性における構造活性相関の解析 (2012)
  • Author(s): 肥塚崇男、染谷信孝、渡辺文太、松井健二、平竹潤
  • Organizer: 日本農芸化学会2012年度大会
  • Place of Presentation: (京都市)京都女子大学
  • Year and Date: 2012-03-23
• [Presentation] Studies on coumarin prenyltransferases involved in tolerance to pathogens and herbivores in lemon (2011)
  • Author(s): Munakata, R., Inoue, T., Koeduka, T., Sasaki, K., Tsurumaru, Y., Sugiyama, A., Uto, Y., Hori, H., Azuma, J., Yazaki, K.
  • Organizer: International Symposium" Strategies of Plants against Global Environmental Change"
  • Place of Presentation: (倉敷市)岡山大学
  • Year and Date: 2011-12-08
• [Presentation] レモンのクマリン基質プレニルトランスフェラーゼの単離と機能解析 (2011)
  • Author(s): 井上剛史、棟方涼介、肥塚崇男、佐々木佳菜子、鶴丸優介、杉山暁史、宇都義浩、堀均、東順一、矢崎一史
  • Organizer: 日本植物細胞分子生物学会2011年度大会
  • Place of Presentation: (福岡市)九州大学
  • Year and Date: 2011-09-07
• [Presentation] レモン由来のプレニル基転移酵素のクローニングと機能解析 (2011)
  • Author(s): 井上剛史、肥塚崇男、佐々木佳菜子、鶴丸優介、杉山暁史、坂本正弘、東順一、矢崎一史
  • Organizer: 日本農芸化学会2011年度大会
  • Place of Presentation: (京都市)京都女子大学
  • Year and Date: 2011-03-27
• [Presentation] プレニル基転移酵素の過剰発現によるトマト形質転換体のフラボノイドの解析 (2011)
  • Author(s): 肥塚崇男、川崎崇、士反伸和、熊野匠人、山本秀明、佐々木佳菜子、原田英美子、杉山暁史、江面浩、葛山智久、矢崎一史
  • Organizer: 日本農芸化学会2011年度大会
  • Place of Presentation: (京都市)京都女子大学
  • Year and Date: 2011-03-27
• [Remarks]
  • URL: http://www.scl.kyoto-u.ac.jp/~hiratake/index-j.html