2011 Fiscal Year Final Research Report
In vitro selection of fluorescent RNAs for in vivo imaging
| Project/Area Number |
22710217
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Living organism molecular science
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| Research Institution | Osaka University |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2011
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| Keywords | RNAアプタマー / 蛍光タンパク質 / 蛍光性RNA |
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| Research Abstract |
In vitro selection provides one of the most powerful strategies for obtaining functional RNA molecules. In this project, we tried to obtain RNA receptors against the synthetic chromophores by in vitro selection to investigate whether synthetic RNA receptor-chromophore complexes can emit fluorescence as other fluorescent proteins do. The selection and evolution technique has produced RNA aptamers that specifically bind small molecules and emit fluorescence only when RNA aptamers bound to synthetic chromophores
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