2011 Fiscal Year Final Research Report
Metabolite profiling in rice plant by Multidimensional NMR-Based Metabolomics
| Project/Area Number |
22710230
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Living organism molecular science
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| Research Institution | National Agriculture and Food Research Organization |
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Principal Investigator |
SEKIYAMA Yasuyo 独立行政法人農業・食品産業技術総合研究機構, 食品総合研究所・食品分析研究領域, 任期付研究員 (60342804)
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| Project Period (FY) |
2010 – 2011
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| Keywords | 機器分析 / メタボローム解析 / 核磁気共鳴法 / 安定同位体標識 |
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| Research Abstract |
^<13> C labeling allows to increase the reliability of metabolite annotation. In an attempt to expand information on metabolite profiles in Oryza sativa, I tried to generate highly^<13> C-labeled rice plants by^<13> CO_2 feeding. The labeling ratio of the labeled plant estimated from the anomeric proton signal of sucrose in the 1^H NMR spectra was 75%. Candidate polar and semipolar metabolites in the leaves were listed from the standard database.
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