2011 Fiscal Year Final Research Report

Metabolite profiling in rice plant by Multidimensional NMR-Based Metabolomics

Research Project

Project/Area Number	22710230
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Living organism molecular science
Research Institution	National Agriculture and Food Research Organization
Principal Investigator	SEKIYAMA Yasuyo 独立行政法人農業・食品産業技術総合研究機構, 食品総合研究所・食品分析研究領域, 任期付研究員 (60342804)
Project Period (FY)	2010 – 2011
Keywords	機器分析 / メタボローム解析 / 核磁気共鳴法 / 安定同位体標識
Research Abstract	^<13> C labeling allows to increase the reliability of metabolite annotation. In an attempt to expand information on metabolite profiles in Oryza sativa, I tried to generate highly^<13> C-labeled rice plants by^<13> CO_2 feeding. The labeling ratio of the labeled plant estimated from the anomeric proton signal of sucrose in the 1^H NMR spectra was 75%. Candidate polar and semipolar metabolites in the leaves were listed from the standard database.

(4 results)