2011 Fiscal Year Final Research Report

Diversity of mycolic acid containing actinomycetes isolated from subtropical region in Japan and its conservation

Research Project

Project/Area Number	22710234
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Resource conservation science
Research Institution	University of Yamanashi
Principal Investigator	YAMAMURA Hideki 山梨大学, 大学院・医学工学総合研究部, 助教 (70516939)
Project Period (FY)	2010 – 2011
Keywords	微生物 / 保全 / 放線菌 / 分類学 / 生態学 / 多様性
Research Abstract	The aim of this study was to reveal the diversity and conservation of mycolic acid containing actinomycete such as Nocardia and Mycobacterium from subtropical area in Japan. Forest and cultivated field soil samples were collected from Iriomote island, Okinawa, Japan. The isolates belonging to the genus Nocardia and Mycobacterium were selectively isolated using sucrose-gradient centrifugation method and Oil-SDS emulsification method. Twelve species of Nocardia and two species of Mycobacterium was detected. Selective isolation method for the genus Rhodococcus was developed as combined use of antibiotics(fradiomycine ; 20 mg/L and novobiocin ; 10 mg/L) and pretreatment of soil with alkaline-SDS solution. Some of isolates were suspected as new species and were deposited in NITE-Biological Resource Center for microbial conservation