The structure and distribution of chlorophylls in photosynthesis

2011 Fiscal Year Final Research Report

• Project/Area Number: 22750017
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Physical chemistry
• Research Institution: Ritsumeikan University
• Principal Investigator: MIZOGUCHI Tadashi 立命館大学, 生命科学部, 准教授 (90343665)
• Project Period (FY): 2010 – 2011
• Keywords: クロロフィル / 色素蛋白質複合体 / 光合成 / 超精密計測

Research Abstract

The structure and distribution of chlorophyll pigments with various long hydrocarbon chains in photosynthetic organisms were investigated by ultra-high-sensitive HPLC at the sub-fmol level.

Research Products

• [Journal Article] Artificially produced [7-formyl]-Chl d functions as an antenna pigment in the photosystem II isolated from the chlorophyllide a oxygenase-expressing Acaryochloris marina (2012)
  • Author(s): Tohru Tsuchiya, Seiji Akimoto, Tadashi Mizoguchi, Kazuyuki Watabe, Hayato Kindo, Tatsuya Tomo, Hitoshi Tamiaki and Mamoru Mimuro
  • Journal Title: Biochim. Biophys. Acta Volume: (in press)
  • Peer Reviewed
• [Journal Article] Photoreduction of zinc 3-acetyl-chlorophyll derivative to prepare chemically stable isobacteriochlorin (2012)
  • Author(s): Hitoshi Tamiaki, Meiyun Xu and Tadashi Mizoguchi
  • Journal Title: Tetrahedron Lett Volume: (in press)
  • Peer Reviewed
• [Journal Article] Isolation and pigment composition of the reaction centers from purple photosynthetic bacterium Rhodopseudomonas palustris species (2012)
  • Author(s): Tadashi Mizoguchi, Megumi Isaji, Jiro Harada and Hitoshi Tamiaki
  • Journal Title: Biochim. Biophys. Acta Volume: 1817 Pages: 395-400
  • Peer Reviewed
• [Journal Article] Metabolic engineering of a chlorophyll d-dominated cyanobacterium Acaryochloris marina : production of a novel chlorophyll species by the introduction of chlorophyllide a oxygenase gene (2012)
  • Author(s): Tohru Tsuchiya, Tadashi Mizoguchi, Seiji Akimoto, Tatsuya Tomo, Hitoshi Tamiaki and Mamoru Mimuro
  • Journal Title: Plant Cell Physiol Volume: 53 Pages: 518-527
  • Peer Reviewed
• [Journal Article] The pigment stoichiometry in a chlorophyll a/c type photosynthetic antenna (2012)
  • Author(s): Ritsuko Fujii, Mamiko Kita, Matsumi Doe, Yoshiro Iinuma, Naohiro Oka, Yuki Takaesu, Tomonori Taira, Masahiko Iha, Tadashi Mizoguchi, Richard J. Cogdell and Hideki Hashimoto
  • Journal Title: Photosynth. Res. Volume: 111 Pages: 165-172
  • Peer Reviewed
• [Journal Article] The stereochemistry of chlorophyll-c_3 from the haptophyte Emiliania huxleyi : The(13^2R)-enantiomers of chlorophylls-c are exclusively selected as the photosynthetically active pigments in chromophyte algae (2011)
  • Author(s): Tadashi Mizoguchi, Yuki Kimura, Taichi Yoshitomi and Hitoshi Tamiaki
  • Journal Title: Biochim. Biophys. Acta Volume: 1807 Pages: 1467-1473
  • Peer Reviewed
• [Journal Article] Temperature-and time-dependent changes in the structure and composition of glycolipids during the growth of the green sulfur photosynthetic bacterium Chlorobium tepidum (2011)
  • Author(s): Tadashi Mizoguchi, Taichi Yoshitomi, Jiro Harada and Hitoshi Tamiaki
  • Journal Title: Biochemistry Volume: 50 Pages: 4504-4512
  • Peer Reviewed
• [Journal Article] Characterization of glycolipids in light-harvesting chlorosomes from the green photosynthetic bacterium Chlorobium tepidum (2011)
  • Author(s): Taichi Yoshitomi, Tadashi Mizoguchi, Michio Kunieda and Hitoshi Tamiaki
  • Journal Title: Bull. Chem. Soc. Jpn. Volume: 84 Pages: 395-402
  • Peer Reviewed
• [Journal Article] In vitro synthesis of bacteriochlorophyll-f and no detection of BChl-f in green photosynthetic bacteria producing BChl-e (2011)
  • Author(s): Hitoshi Tamiaki, Jun Komada, Michio Kunieda, Kazuhiro Fukai, Taichi Yoshitomoi, Jiro Harada and Tadashi Mizoguchi
  • Journal Title: Photosynth. Res. Volume: 107 Pages: 133-138
  • Peer Reviewed
• [Journal Article] Biosynthesis of unnatural bacteriochlorophyll c derivatives esterified with α,ω-diols in the green sulfur photosynthetic bacterium Chlorobaculum tepidum (2011)
  • Author(s): Risato Nishimori, Tadashi Mizoguchi, Hitoshi Tamiaki, Shigenori Kashimura and Yoshitaka Saga
  • Journal Title: Biochemistry Volume: 50 Pages: 7756-7764
  • Peer Reviewed
• [Journal Article] Reduction of vinyl groups in naturally occurring chlorophylls (2011)
  • Author(s): Hitoshi Tamiaki, Daisuke Takekoshi and Tadashi Mizoguchi
  • Journal Title: Bioorg. Med. Chem Volume: 19 Pages: 52-57
  • Peer Reviewed
• [Journal Article] X-ray crystal structure of the light-independent protochlorophyllide reductase (2010)
  • Author(s): Norifumi Muraki, Jiro Nomata, Kozue Ebata, Tadashi Mizoguchi, Tomoo Shiba, Hitoshi Tamiaki, Genji Kurisu and Yuichi Fujita
  • Journal Title: Nature Volume: 465 Pages: 110-114
  • Peer Reviewed
• [Journal Article] Exclusive observation of the 13^2-R epimer in a diatom Chaetoseros calcitrans (2010)
  • Author(s): Tadashi Mizoguchi, Yuki Kimura and Hitoshi Tamiaki
  • Journal Title: Photochem. Photobiol. Volume: 86 Pages: 311-315
  • Peer Reviewed
• [Journal Article] Synthesis of^<18> O-labelled photosynthetically acitive chlorophylls at the 3-or 7-carbonyl group with high regioselectivity (2010)
  • Author(s): Hidetada Morishita, Tadashi Mizoguchi and Hitoshi Tamiaki
  • Journal Title: Photosynth. Res. Volume: 105 Pages: 257-263
  • Peer Reviewed
• [Presentation] 紅色光合成細菌Rhodopseudomonas palustris種からの反応中心の単離とそのクロロフィル色素組成 (2012)
  • Author(s): 溝口正、伊佐治恵、原田二朗、民秋均
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都産業大学(京都府)
  • Year and Date: 2012-03-18
• [Presentation] Chemistry of naturally occurring chlorophylls-c isolated from chromophyta algae (2011)
  • Author(s): H. Tamiaki, Y. Kimura, T. Mizoguchi
  • Organizer: 光合成器官のテトラピロール光受容体に関する国際会議(ICTPPO2012)
  • Place of Presentation: ベルリン(ドイツ)
  • Year and Date: 2011-07-27
• [Presentation] 緑色硫黄細菌の糖脂質解析:生育環境に応答した構造変換 (2011)
  • Author(s): 溝口正、吉冨太一、原田二朗、民秋均
  • Organizer: 第19回光合成の色素系と反応中心に関するセミナー(2011)
  • Place of Presentation: 大阪大学豊中キャンパス(大阪府)
  • Year and Date: 2011-07-10
• [Presentation] The stereochemistry of porphyrinoid chlorophylls-c : The(13^2R)-enantiomers are exclusively selected as the photosynthetically active pigments in chromophyte algae (2011)
  • Author(s): T. Mizoguchi, Y. Kimura, T. Yoshitomi, H. Tamiaki
  • Organizer: 超分子系と配位化合物の光化学と光生物学に関する国際研究集会(SNCPP11)
  • Place of Presentation: 立命館大学(滋賀県)
  • Year and Date: 2011-06-11
• [Presentation] 光合成系における機能性生体分子の精密構造解析 (2010)
  • Author(s): 溝口正
  • Organizer: 光合成学会若手の会第3回セミナー
  • Place of Presentation: 東京大学本郷キャンパス(東京都)
  • Year and Date: 2010-10-16
• [Presentation] Reduction manner of geranylgeranyl group in the green sulfur bacterium Chlorobaculum tepidum (2010)
  • Author(s): J. Harada, S. Miyago, T. Mizoguchi, H. Tamiaki, H. Oh-oka
  • Organizer: 第15回国際光合成会議(PS2010)
  • Place of Presentation: 北京(中国)
  • Year and Date: 2010-08-24
• [Presentation] Stereochemistry of natural chlorophylls-c from diatoms and brown algae (2010)
  • Author(s): H. Tamiaki, Y. Kimura, T. Mizoguchi
  • Organizer: 第6回ポルフィリン-フタロシアニン国際会議
  • Place of Presentation: ニューメキシコ(アメリカ)
  • Year and Date: 2010-07-06
• [Presentation] The structure of 17-propionate on(bacterio) chlorophylls : Biological implication of their long esterifying chains in light-harvesting antenna systems (2010)
  • Author(s): T. Mizoguchi, H. Tamiaki
  • Organizer: 超分子系と配位化合物の光化学と光生物学に関する国際研究集会(SNCPP10)
  • Place of Presentation: 立命館大学(滋賀県)
  • Year and Date: 2010-06-12