Development of functional cell array for long-term survival and recovery of tissues

2011 Fiscal Year Final Research Report

• Project/Area Number: 22750066
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Analytical chemistry
• Research Institution: The University of Tokyo
• Principal Investigator: YOSHIMOTO Keitaro 東京大学, 大学院・総合文化研究科, 准教授 (60392172)
• Project Period (FY): 2010 – 2011
• Keywords: 細胞分離 / 細胞培養皿 / 核酸

Research Abstract

In order to develop the new method for cell recovery, a high-performance magnetic beads for biotinyl-compounds recovery was developed. In addition, assesment of cell attachemenct on DNA immobilized surface, spheroid culture of primary hepatocytes, and co-culture system of cancer spheroid were demonstrated.

Research Products

• [Journal Article] Long-term survival and functional maintenance of hepatocytes by using a microfabricated cellarray (2012)
  • Author(s): Ikeda, Y., Jomura, T., Horiuchi, U., Saeki, J., Yoshimoto, K., Ikeya, T., Nagasaki, Y
  • Journal Title: Colloids and Surfaces B : Biointerfaces Pages: 97-100
• [Journal Article] Improvement of the thermal stability of streptavidin immobilized on magnetic beads by the construction of a mixed-poly(ethylene glycol) tethered-chain Layer (2011)
  • Author(s): Kubota, M., Yoshimoto, K., Yuan, X., Nagasaki, Y
  • Journal Title: Polymer Journal Volume: 43 Pages: 493-496
• [Presentation] 高分子修飾表面の界面力-生体高分子・細胞に与える不思議な環境 (2012)
  • Author(s): 吉本敬太郎
  • Organizer: 第12回表面力セミナー
  • Place of Presentation: 東北大学
  • Year and Date: 2012-03-03
• [Presentation] 3D Cell Co-culture System on Hydrogel Micro-Patterned Surface (2011)
  • Author(s): Keitaro Yoshimoto
  • Organizer: MRS Fall Meeting
  • Place of Presentation: Boston, USA
  • Year and Date: 2011-11-29
• [Presentation] 培養細胞の構造制御と高機能化-細胞も3Dの時代 (2011)
  • Author(s): 吉本敬太郎
  • Organizer: 第12回機能構造と分析化学シンポジウム
  • Place of Presentation: 東北大学
  • Year and Date: 2011-11-19
• [Presentation] Fab'/mixed-PEG co-immobilized gold surface-The role of a PEG tethered layer in molecular recognition events- (2011)
  • Author(s): Yukio Nagasaki, Motohiko Nishio, Keitaro Yoshimoto
  • Organizer: ICTF15
  • Place of Presentation: Fukuoka
  • Year and Date: 2011-11-08
• [Presentation] 分子認識の選択性を高めるソフト界面設計 (2011)
  • Author(s): 吉本敬太郎,長崎幸夫
  • Organizer: 日本分析化学会第60年会
  • Place of Presentation: 名古屋大学
  • Year and Date: 2011-09-14
• [Presentation] Structural Analysis and Molecular Recognition Behavior of Bio-macromolecular/Polymer Hybrid Interface (2011)
  • Author(s): Keitaro Yoshimoto
  • Organizer: ICAS2011
  • Place of Presentation: Kyoto International Conference Center, Kyoto, Japan
  • Year and Date: 2011-05-24
• [Presentation] 生体高分子構造の空間制御に基づく高性能診断法の開発 (2010)
  • Author(s): 吉本敬太郎
  • Organizer: 日本分析化学会第59年会
  • Place of Presentation: 東北大学
  • Year and Date: 2010-09-16
• [Presentation] 胎生肝細胞の長期培養と分化誘導法 (2010)
  • Author(s): 吉本敬太郎,小島綾太,長崎幸夫
  • Organizer: 第59回高分子学会年次大会
  • Place of Presentation: 横浜
  • Year and Date: 2010-05-28