2011 Fiscal Year Final Research Report
Development of Simultaneous detection for Bacteria, Virus, and Toxin using Microchip with Ion-channel Sensor
| Project/Area Number |
22750068
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Analytical chemistry
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| Research Institution | University of Toyama |
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Principal Investigator |
HIDEKI Kuramitz 富山大学, 大学院・理工学研究部(理学), 准教授 (70397165)
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| Project Period (FY) |
2010 – 2011
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| Keywords | 電気化学分析 / 大腸菌 / 毒素タンパク質 / マイクロ流路 / 磁性粒子 |
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| Research Abstract |
The aim of this study is the development for the microchip devise with ion-channel sensor for simultaneous detection of bacteria, virus, and protein toxin. The development of detecting units for cholera toxin, MS2, and E-coli were successively achieved by constructing the antibody assembled membrane that acts as the ion-channel for ferrocyanide on the electrode surface. As the separating units, the separation of E-coli bound/unbound magnetic microbeads(1μm) was investigated using the pinched flow fractionation microchip. The determination of 5×10^2 cfu/mL E-coli was achieved by this method.
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[Journal Article]2012
Author(s)
H. Kuramitz, Y. Mawatari, M. Ikeuchi, O. Kutomi, N. Hata, S. Taguchi, and K. Sugawara
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Journal Title
Analytical Sciences
Volume: 28
Pages: 77-81
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[Journal Article]2012
Author(s)
M. Fukushima, Y. Mizutani, S. Maeno, Q. Zhu, H. Kuramitz, and S. Nagao
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Journal Title
Molecules
Volume: 17
Pages: 48-60
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[Journal Article]2011
Author(s)
K. Sazawa, M. Tachi, T. Wakimoto, T. Kawakami, N. Hata, S. Taguchi and H. Kuramitz
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Journal Title
International Journal of Environmental Research and Public Health
Volume: 8
Pages: 1655-1670
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[Journal Article]2011
Author(s)
H. Kuramitz, S. Miyagaki, E. Ueno, N. Hata, S. Taguchi, and K. Sugawara
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Journal Title
Analyst
Volume: 136
Pages: 2373-2378
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[Presentation]2011
Author(s)
福嶋正巳・水谷祐介・前野翔平・倉光英樹・長尾誠也
Organizer
第27回日本腐植物質学会講演会
Place of Presentation
金沢大学
Year and Date
20111117-18
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[Presentation]2011
Author(s)
K. Sazawa, W. Ohura, Y. Furuhashi, N. Hata, S. Taguchi, M. Fukushima, and H. Kuramitz
Organizer
The 4^<th> IWA-ASPIRE Conference & Exhibition
Place of Presentation
Tokyo, Japan
Year and Date
20111002-06
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[Presentation]2011
Author(s)
T. Okazaki, W. Wang, H. Kuramitz, N. Hata, and S. Taguchi
Organizer
The 4^<th> IWA-ASPIRE Conference & Exhibition
Place of Presentation
Tokyo, Japan
Year and Date
20111002-06
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[Presentation]2011
Author(s)
N. Hata, T. Nakajima, K. Nakagawa, M. Nakamura, H. Kuramitz, S. Taguchi, and H. Chiba
Organizer
The 4^<th> IWA-ASPIRE Conference & Exhibition
Place of Presentation
Tokyo, Japan
Year and Date
20111002-06
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[Presentation]2011
Author(s)
N. Hata, S. Terashima, R. Yasui, H. Kuramitz, and S. Taguchi
Organizer
The 4^<th> IWA-ASPIRE Conference & Exhibition
Place of Presentation
Tokyo, Japan
Year and Date
20111002-06
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[Presentation]2011
Author(s)
S. Taguchi, K. Murai, Y. Hayakawa, S. Tamizu, M. Kuwata, Y. Katayama, N. Hata, and H. Kuramitz
Organizer
The 4^<th> IWA-ASPIRE Conference & Exhibition
Place of Presentation
Tokyo, Japan
Year and Date
20111002-06
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[Presentation]2011
Author(s)
T. Wakimoto, K. Sazawa, N. Hata, S. Taguchi, and H. Kuramitz
Organizer
3rd International Workshop on "Wild Fire and Carbon Management in Peat-Forest in Indonesia"
Place of Presentation
Palangka Raya, Indonesia
Year and Date
20110920-24
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[Presentation]2011
Author(s)
M. Ikeuchi, H. Kuramitz, N. Hata, S. Taguchi, and K. Sugawara
Organizer
62^<nd> Annual Meeting of the International Society of Electrochemistry
Place of Presentation
Niigata, Japan
Year and Date
20110911-16
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[Presentation]2010
Author(s)
K. Sazawa, H. Kuramitz, N. Hata, and S. Taguchi
Organizer
The 17^<th> Asian Symposium on Ecotechnology(ASET17)
Place of Presentation
Toyama, Japan
Year and Date
20101111-13
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[Presentation]2010
Author(s)
H. Kuramitz, K. Sazawa, N. Hata, and S. Taguchi
Organizer
2nd International Workshop on "Wild Fire and Carbon Management in Peat-Forest in Indonesia"
Place of Presentation
Palangka Raya, Indonesia
Year and Date
20100928-29
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[Presentation]2010
Author(s)
K. Sazawa, H. Kuramitz, N. Hata, and S. Taguchi
Organizer
2nd International Workshop on "Wild Fire and Carbon Management in Peat-Forest in Indonesia"
Place of Presentation
Palangka Raya, Indonesia
Year and Date
20100928-29
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[Presentation]2010
Author(s)
W. R. Heineman, H. B. Halsall, C. J. Seliskar, H. Kuramitz
Organizer
The 61^<st> Annual Meeting of the International Society of Electrochemistry
Place of Presentation
Nice, France
Year and Date
20100926-1001
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