2011 Fiscal Year Final Research Report
Development of Microchip Electrophoresis.Mass Spectrometry Analysis of Biomolecules Using Nanoparticles
| Project/Area Number |
22750069
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Analytical chemistry
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| Research Institution | Hirosaki University (2011)
Kyoto University (2010) |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2011
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| Keywords | マイクロチップ電気泳動 / 質量分析 / 有機ナノ結晶 / 酸化チタンナノ粒子 / キラル分離 / リン酸化ペプチド |
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| Research Abstract |
In this study, nanoparticles were applied to capillary electrophoresis (CE) and capillary electrochromatography (CEC) analysis of enantiomers and phosphopeptides, respectively. For the CEC analysis of enantiomers, cinchonidine (CCND) nanocrystals were prepared by an emusion method to immobilize onto the inner surface of fused silica capillaries and microchips. By using the CCND nanocrystals stationary phase, the CEC enantioseparations of racemic amino acids were successfully achieved. In the CE analysis of phosphopeptides, on the other hand, an on-line preconcentration technique using partially injected titania nanoparticles was developed to improve the detection sensitivity. By injecting a short plug of the titania dispersion into the separation column, ATP and phosphopeptide released from ss-casein were well concentrated
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