Application of Transient Trapping to Capillary Electrophoresis-Mass Spectrometry for High Performance Bioanalysis

2011 Fiscal Year Final Research Report

• Project/Area Number: 22750070
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Analytical chemistry
• Research Institution: Kyoto University
• Principal Investigator: SUEYOSHI Kenji 京都大学, 大学院・工学研究科, 助教 (70552660)
• Project Period (FY): 2010 – 2011
• Keywords: 分離分析 / 質量分析法 / オンライン試料濃縮 / 動電クロマトグラフィー

Research Abstract

Application of on-line sample preconcentration technique named transient trapping to capillary electrophoresis. mass spectrometry improved both a resolution and sensitivity in electrophoretic analyses for biogenic compounds.

Research Products

• [Journal Article] Recent progress of on-line sample preconcentration techniques in microchip electrophoresis (2012)
  • Author(s): Kitagawa, F.; Kawai, T.; Sueyoshi, K.; Otsuka, K.
  • Journal Title: Analytical Science Volume: 28 Pages: 85-93
  • DOI: DOI:10.2116/analsci.28.85.
  • Peer Reviewed
• [Journal Article] Highly sensitive oligosaccharide analysis in capillary electrophoresis using large-volume sample stacking with an electroosmotic flow pump (2012)
  • Author(s): Kawai, T.; Watanabe, M.; Sueyoshi, K.; Kitagawa, F.; Otsuka, K.
  • Journal Title: Journal of Chromatography A Volume: 1232 Pages: 52-58
  • DOI: DOI:10.1016/j.chroma.2011.09.032.
  • Peer Reviewed
• [Journal Article] Recent progress of on-line combination of preconcentration device with microchip electrophoresis (2012)
  • Author(s): Sueyoshi, K.
  • Journal Title: Chromatography Volume: 33 Pages: 25-33
  • Peer Reviewed
• [Journal Article] Hydrophobic labeling of amino acids : transient trapping. capillary micro-chip electrophoresis (2011)
  • Author(s): Sueyoshi, K.; Hashiba, K.; Kawai, T.; Kitagawa, F.; Otsuka, K.
  • Journal Title: Electrophoresis Volume: 32 Pages: 1233-1240
  • DOI: DOI:10.1002/elps.201000567.
  • Peer Reviewed
• [Journal Article] マイクロチップ電気泳動におけるオンライン試料濃縮技術 (2011)
  • Author(s): 北川文彦, 末吉健志, 大塚浩二
  • Journal Title: ぶんせき Volume: 3 Pages: 143-149
  • Peer Reviewed
• [Journal Article] One-step preparation of amino-PEG modified poly(methyl methacrylate) microchips for electrophoretic separation of biomolecules (2010)
  • Author(s): Kitagawa, F.; Kubota, K.; Sueyoshi, K.; Otsuka, K.
  • Journal Title: Journal of Pharmaceutical and Biomedical Analysis Volume: 53 Pages: 1272-1277
  • DOI: DOI:10.1016/j.jpba.2010.07.008.
  • Peer Reviewed
• [Journal Article] Microchip electrophoresis of oligosaccharides using large-volume sample stacking with electroosmotic flow pump in single channe (2010)
  • Author(s): Kawai, T.; Sueyoshi, K.; Kitagawa, F.; Otsuka, K.
  • Journal Title: Analytical Chemistry Volume: 82 Pages: 6504-6511
  • DOI: DOI:10.1021/ac1008145.
  • Peer Reviewed
• [Presentation] キチン修飾PDMS製マイクロチップを用いるタンパク質の電気泳動分析 (2012)
  • Author(s): 末吉健志, 堀祐輔, 大塚浩二
  • Organizer: 日本化学会第92春季年会
  • Place of Presentation: 慶應義塾大学日吉キャンパス・矢上キャンパス,東京
  • Year and Date: 20120325-28
• [Presentation] Capillary/Microchip Gel Electrophoresis Using Alginate Hydrogel as a Sieving Medium (2012)
  • Author(s): Kenji Sueyoshi, Masahiro Ikawa, Fumihiko Kitagawa, Koji Otsuka
  • Organizer: 27th International Symposium on Microscale Bioseparations(MSB2012)
  • Place of Presentation: Starling Hotel and Conference Center, Geneva, Switzerland.
  • Year and Date: 20120212-16
• [Presentation] Transient Trapping in Enantioseparation for High-Sensitive Detection (2011)
  • Author(s): Kenji Sueyoshi, Hiroshi Koino, Fumihiko Kitagawa, Koji Otsuka
  • Organizer: 11th Asia Pacific International Symposium on Microscale Separations and Analysis(APCE2011)
  • Place of Presentation: University of Tasmania, Tasmania, Australia
  • Year and Date: 20111127-30
• [Presentation] ミクロスケール電気泳動の高機能化・高感度化に関する研究 (2011)
  • Author(s): 末吉健志
  • Organizer: 第22回クロマトグラフィー科学会議
  • Place of Presentation: 東北大学,仙台
  • Year and Date: 20111020-22
• [Presentation] アルギン酸-カルシウムヒドロゲルを分離媒体として用いるマイクロチップ電気泳動分析法の開発 (2011)
  • Author(s): 井川真宏, 末吉健志, 北川文彦, 大塚浩二
  • Organizer: 第55回日本学術会議材料工学連合講演会
  • Place of Presentation: 京都教育文化センター,京都
  • Year and Date: 20111019-21
• [Presentation] アルギン酸ヒドロゲルを用いたマイクロチップ電気泳動分析法の開発 (2011)
  • Author(s): 井川真宏, 末吉健志, 北川文彦, 大塚浩二
  • Organizer: 日本分析化学会第60年会
  • Place of Presentation: 名古屋大学・東山キャンパス,名古屋
  • Year and Date: 20110914-16
• [Presentation] Rapid and sensitive analysis using transient trapping in microchip electrophoresis (2011)
  • Author(s): Kenji Sueyoshi, Fumihiko Kitagawa, Koji Otsuka
  • Organizer: The 14th Asian Chemical Congress(14ACC)
  • Place of Presentation: The Queen Sirikit National Convention Center, Bangkok, Thai
  • Year and Date: 20110905-08
• [Presentation] On-line Preconcentration of Proteins in Floating Electrode-integrated Microchannel (2011)
  • Author(s): Kenji Sueyoshi, Kana Tanigawa, Fumihiko Kitagawa, Koji Otsuka
  • Organizer: The International Symposium on Microchemistry and Microsystems(ISMM 2011)
  • Place of Presentation: Hotel Seoul Kyoyuk Munhwa Hoekwan, Seoul, Korea
  • Year and Date: 20110602-04
• [Presentation] Nafion Modified PMMA Microchips for Rapid Electrophoretic Analysis (2011)
  • Author(s): Kenji Sueyoshi, Mariko Seno, Fumihiko Kitagawa, Koji Otsuka
  • Organizer: The International Symposium on Microchemistry and Microsystems(ISMM 2011)
  • Place of Presentation: Hotel Seoul Kyoyuk Munhwa Hoekwan, Seoul, Korea
  • Year and Date: 20110602-04
• [Presentation] Rapid and sensitive electrophoretic analysis in transient trapping. micellar electrokinetic chromato graphy (2011)
  • Author(s): Kenji Sueyoshi, Hiroshi Koino, Kota Hashiba, Fumihiko Kitagawa, Koji Otsuka
  • Organizer: IUPAC International Congress onAnalytical Sciences 2011(ICAS2011)
  • Place of Presentation: Kyoto International Conference Center, Kyoto, Japan
  • Year and Date: 20110522-26
• [Presentation] Transient trapping in bioanalysis for high-sensitive detection (2011)
  • Author(s): Ryuta Tanaka, Kenji Sueyoshi, Fumihiko Kitagawa, Koji Otsuka
  • Organizer: IUPAC International Congress on Analytical Sciences 2011(ICAS2011)
  • Place of Presentation: Kyoto International Conference Center, Kyoto, Japan
  • Year and Date: 20110522-26
• [Presentation] Stable coating using Nafion thin membrane for fast electroosmotic flow in polymer microchips (2011)
  • Author(s): Kenji Sueyoshi, Mariko Seno, Fumihiko Kitagawa, Koji Otsuka
  • Organizer: IUPAC International Congress on Analytical Sciences 2011(ICAS2011)
  • Place of Presentation: Kyoto International Conference Center, Kyoto, Japan
  • Year and Date: 20110522-26
• [Presentation] トランジェント-トラッピング法によるミクロスケール電気泳動分析の高感度化(3) (2011)
  • Author(s): 末吉健志, 恋野寛嗣, 田中隆太, 北川文彦, 大塚浩二
  • Organizer: 日本化学会第91春季年会
  • Place of Presentation: 神奈川大学横浜キャンパス,横浜
  • Year and Date: 20110326-29
• [Presentation] Rapid and sensitive analysis based on transient trapping in capillary electrophoresis (2010)
  • Author(s): Kenji Sueyoshi, Kota Hashiba, Ryuta Tanaka, Fumihiko Kitagawa, Koji Otsuka
  • Organizer: 2010 International Chemical Congress of Pacific Basin Societies(PACIFICHEM 2010)
  • Place of Presentation: Honolulu, HI, USA
  • Year and Date: 20101215-20
• [Presentation] Rapid and sensitive analysis based on transient trapping in microchip electrophoresis (2010)
  • Author(s): Kenji Sueyoshi, Kota Hashiba, Ryuta Tanaka, Fumihiko Kitagawa, Koji Otsuka
  • Organizer: 2010 International Chemical Congress of Pacific Basin Societies(PACIFICHEM 2010)
  • Place of Presentation: Honolulu, HI, USA
  • Year and Date: 20101215-20
• [Presentation] トランジェント-トラッピング法によるミクロスケール電気泳動分析の高感度化(2) (2010)
  • Author(s): 末吉健志, 恋野寛嗣, 田中隆太, 橋場皇太, 北川文彦, 大塚浩二
  • Organizer: 第30回キャピラリー電気泳動シンポジウム
  • Place of Presentation: 長良川国際会議場,岐阜
  • Year and Date: 20101115-17
• [Presentation] トランジェント-トラッピング法による光学異性体分析の高感度化(3 (2010)
  • Author(s): 末吉健志, 恋野寛嗣, 橋場皇太, 北川文彦, 大塚浩二
  • Organizer: 第21回クロマトグラフィー科学会議
  • Place of Presentation: 武庫川女子大学薬学部,西宮
  • Year and Date: 20101021-23
• [Presentation] Fluorescence imaging analysis of transient trapping-microchip micellar electrokinetic chromatography (2010)
  • Author(s): Kenji Sueyoshi, Fumihiko Kitagawa, Koji Otsuka
  • Organizer: 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences(microTAS2010)
  • Place of Presentation: Groningen, The Netherlands
  • Year and Date: 20101003-07
• [Presentation] トランジェント-トラッピング法による光学異性体分析の高感度化 (2010)
  • Author(s): 末吉健志, 恋野寛嗣, 橋場皇太, 北川文彦, 大塚浩二
  • Organizer: 日本分析化学会第59年会
  • Place of Presentation: 東北大学川内北キャンパス,仙台
  • Year and Date: 20100915-17
• [Presentation] トランジェント-トラッピング法によるミクロスケール電気泳動分析の高感度化 (2010)
  • Author(s): 末吉健志, 恋野寛嗣, 北川文彦, 大塚浩二
  • Organizer: 東京コンファレンス2010
  • Place of Presentation: 幕張メッセ国際会議場,千葉
  • Year and Date: 20100901-03
• [Presentation] Highly effective sample preconcentration and separation based on transient trapping in microscale electrophoresis (2010)
  • Author(s): Kenji Sueyoshi, Kota Hashiba, Ryuta Tanaka, Fumihiko Kitagawa, Koji Otsuka
  • Organizer: 35th International Symposium on High Performance Liquid Phase Separations and Related Techniques(HPLC 2010), The Hynes Convention Center & Sheraton Boston Hotel
  • Place of Presentation: Boston, MA, USA.
  • Year and Date: 20100619-24
• [Presentation] マイクロチップ電気泳動における新規オンライン試料濃縮法:トランジェント-トラッピング法の開発(7) (2010)
  • Author(s): 末吉健志, 橋場皇太, 田中隆太, 北川文彦, 大塚浩二
  • Organizer: 第21回化学とマイクロ・ナノシステム研究会
  • Place of Presentation: 東京大学本郷キャンパ
  • Year and Date: 20100610-11
• [Presentation] 磁性流体の利用によるマイクロチップ電気泳動の高機能化(5) (2010)
  • Author(s): 末吉健志, 寒川隆史, 北川文彦, 大塚浩二
  • Organizer: 第21回化学とマイクロ・ナノシステム研究会
  • Place of Presentation: 東京大学本郷キャンパス
  • Year and Date: 20100610-11
• [Presentation] トランジェント-トラッピング法によるMEKCの高感度化(17) (2010)
  • Author(s): 末吉健志, 橋場皇太, 田中隆太, 北川文彦, 大塚浩二
  • Organizer: 第17回クロマトグラフィーシンポジウム
  • Place of Presentation: 広島県情報プラザ
  • Year and Date: 20100603-05
• [Presentation] トランジェント-トラッピング法によるMEKCの高感度化(18) (2010)
  • Author(s): 末吉健志, 田中隆太, 橋場皇太, 北川文彦, 大塚浩二
  • Organizer: 第17回クロマトグラフィーシンポジウム
  • Place of Presentation: 広島県情報プラザ
  • Year and Date: 20100603-05
• [Presentation] Electrokinetic filtering on magnetic fluid nano-pillar for high-sensitive electrophoretic analysis (2010)
  • Author(s): Takafumi Samukawa, Kenji Sueyoshi, Fumihiko Kitagawa, Koji Otsuka
  • Organizer: International Symposium on Microchemistry and Microsystems 2010(ISMM 2010), The Hong Kong University of Science and Tecnology
  • Place of Presentation: Hong Kong, China
  • Year and Date: 20100528-30
• [Presentation] トランジェント-トラッピング法によるMEKCの高感度化(15) (2010)
  • Author(s): 末吉健志, 橋場皇太, 田中隆太, 北川文彦, 大塚浩二
  • Organizer: 第71回分析化学討論会
  • Place of Presentation: 島根大学,松江キャンパス
  • Year and Date: 20100515-16
• [Remarks]
  • URL: http://anchem.mc.kyoto-u.ac.jp/