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2012 Fiscal Year Final Research Report

Regulation of protein function in living cells by using a unique enzyme reaction

Research Project

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Project/Area Number 22750071
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Analytical chemistry
Research InstitutionKyushu Institute of Technology

Principal Investigator

SUEDA Shinji  九州工業大学, 大学院・情報工学研究院, 准教授 (00325581)

Project Period (FY) 2010 – 2012
Keywordsタンパク質機能制御 / ビオチン化反応 / 膜タンパク質 / 細胞膜
Research Abstract

Biotinylation reaction from archaeon Sulfolobus tokodaii has a unique characteristic that the biotin ligase forms a stable complex with its biotinylated substrate protein. In the present work, by using this unique biotinylation reaction, we attempted to construct the system that enables the regulation of the functions of the target proteins in living cells. As a result of the study , we have succeeded in the regulation of localization of a target protein in living cells.

  • Research Products

    (5 results)

All 2013 2012 2011

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (3 results)

  • [Journal Article] An SH2 domain-based tyrosine kinase assay using biotin ligase modified with a terbium(III) complex2013

    • Author(s)
      末田慎二,新北幸,草場壮
    • Volume
      Vol. 29
    • Pages
      491-497
    • DOI

      DOI: 10.2116/analsci.29.491

    • Peer Reviewed
  • [Journal Article] Site-specific labeling of proteins by using biotin protein ligase conjugated with fluorophores2011

    • Author(s)
      末田慎二,米田佐和子,林秀樹
    • Volume
      Vol.12
    • Pages
      1367-1375
    • DOI

      DOI: 10.1002/cbic.201000738

    • Peer Reviewed
  • [Presentation] 膜タンパク質とビオチンリガーゼの融合体を利用した細胞内タンパク質の局在制御2012

    • Author(s)
      有須田一馬、末田慎二
    • Organizer
      日本分析化学会第61 年会
    • Place of Presentation
      金沢大学角間キャンパス(石川県)
    • Year and Date
      2012-09-19
  • [Presentation] 特異な酵素反応を利用した細胞内タンパク質の機能制御システムの構築に関する検討2012

    • Author(s)
      有須田一馬、末田慎二
    • Organizer
      第49回化学関連支部合同九州大会
    • Place of Presentation
      北九州国際会議場(福岡県)
    • Year and Date
      2012-06-30
  • [Presentation] 蛍光性ビオチンリガーゼを利用した生細胞系でのタンパク質の蛍光ラベル化技術の開発2011

    • Author(s)
      末田慎二、米田佐和子、山本千裕
    • Organizer
      日本分析化学会第60 年会
    • Place of Presentation
      名古屋大学東山キャンパス(愛知県)
    • Year and Date
      2011-09-24

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Published: 2014-08-29  

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