2011 Fiscal Year Final Research Report
Development of a highly sensitive visual immunoassay method based on DNA amplification and gold nanoparticles.
| Project/Area Number |
22750076
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Analytical chemistry
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| Research Institution | Japan Women's University |
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Principal Investigator |
SATO Kae 日本女子大学, 理学部, 准教授 (40373310)
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| Project Period (FY) |
2010 – 2011
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| Keywords | DNA / RCA / ビーズ |
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| Research Abstract |
Immunoassay is one of the most important analytical methods because of its specificity. To simplify the analysis, a new method based on highly sensitive visual test is strongly required. We established fundamental technologies for immunoassays using enzymes and conjugates of inorganic materials (gold nanoparticle) and biomolecules (DNA). We investigated to develop solid substrates used as efficient reaction fields, make DNA-modified antibodies, and optimize the reactions of gold nanoparticles and amplified products of DNA samples.
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