2011 Fiscal Year Final Research Report
Single-Molecules Tunnel-Current Imaging for DNA duplex by using STM.
| Project/Area Number |
22750078
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Analytical chemistry
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| Research Institution | Osaka University |
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Principal Investigator |
OHSHIRO Takahito 大阪大学, 産業科学研究所, 特任助教(常勤) (10462665)
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| Project Period (FY) |
2010 – 2011
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| Keywords | 表面分析 / DNA / 走査型トンネル顕微鏡 / 電気計測 |
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| Research Abstract |
I developed methods on single-molecule tunnel-current identification with STM and nanogap-electrode. By using these methods, the electron-conductance values were determined for perfect-matched and miss-matched DNA duplexes, and single DNA/ RNA nucleotides, and post-transcriptional modified-nucleotides. Based on these relative conductance values, the sequence of oligonucleotides was successfully determined. In addition, methodologies, for a molecule-control by using nanopore, molecule-recognition by using photonic waveguide, and a construction of DNA-based nanostructures, were also developed.
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[Journal Article] Synthsis, Structure, and Biological Activity of Dumbbell-Shaped Nanocircular RNAs for RNA Interference2011
Author(s)
N. Abe, H. Abe, C. Nagai, M. Harada, H. Hatakeyama, H. Harashima, T. Ohshiro, M. Nishihara, K. Furukawa, M. Maeda, S. Tsuneda, Y. Ito
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Journal Title
Bioconjugate Chemistry
Volume: 22巻
Pages: 2082-2092
Peer Reviewed
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