Kinetic-based analysis for quantized folding of closed-circular DNA

2012 Fiscal Year Final Research Report

• Project/Area Number: 22750098
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Polymer chemistry
• Research Institution: The University of Tokyo
• Principal Investigator: OSADA Kensuke 東京大学, 大学院・工学系研究科, 准教授 (10396947)
• Project Period (FY): 2010 – 2012
• Keywords: 生体関連高分子 / DNA 凝縮

Research Abstract

Mechanistic analysis for structural formation of polyplex micelles as gene delivery system was demonstrated. Main object of this work was placed on understanding principle factors to determine the folding number of plasmid DNA (pDNA) within the polyplex micelles, where pDNA is packaged by highly regulated folding manner, namely quantized folding scheme. The research performed on a basis of kinetic analysis approved that folding process of pDNA is consistently described in the same way as kinetics of polymer crystallization. The deeper understanding on the structure formation of polyplex micelles enables secure molecular design for promoting effective gene delivery carriers.

Research Products

• [Journal Article] Bioactivepolymeric metallosomesself-assembled through blockcopolymer-metal complexation. (2012)
  • Author(s): K. Osada, H. Cabral, Y. Mochida, S. -ELee, K. Nagata, T. Matsuura, M.Yamamoto, Y. Anraku, A. Kishimura, N.Nishiyama, K. Kataoka
  • Journal Title: J. Am.Chem. Soc. Volume: 134(32) Pages: 13172-13175
  • DOI: DOI:10.1021/ja304615y
  • Peer Reviewed
• [Journal Article] Effective transgene expression without toxicity by intraperitoneal administration of PEG-detachable polyplex micelles in mice with peritoneal dissemination. (2012)
  • Author(s): M. Kumagai, S. Shimoda, R. Wakabayashi, Y. Kunisawa, T. Ishii, K. O sa d a, K. Itaka, N. Nishiyama, K. Kataoka, K. Nakano
  • Journal Title: J. Control. Release Volume: 160(3) Pages: 542-551
  • DOI: DOI:10.1016/j.jconrel.2012.03.021
  • Peer Reviewed
• [Journal Article] Targeted polymeric micelles for siRNA treatment of experimental cancer by intravenous injection. (2012)
  • Author(s): R. J. Christie, Y. Matsumoto, K.Miyata, T. Nomoto, S. Fukushima, K. O s a da , J. Halnaut, F. Pittella, H. -J. Kim, N. Nishiyama, K. Kataoka
  • Journal Title: ACS Nano Volume: 6(6) Pages: 5174-5189
  • DOI: DOI:10.1021/nn300942b
  • Peer Reviewed
• [Journal Article] PEGylated polyplex with optimized PEG shielding enhances gene introduction in lungs by minimizing inflammatory responses (2012)
  • Author(s): S. Uchida, K. Itaka, Q. Chen, K . Os a da ,D. Kobayashi, H. Oshima, S. Ikeda, R.J. Christie, K. Itaka, K. Kataoka
  • Journal Title: Mol Ther Volume: 20(6) Pages: 1196-1203
  • DOI: DOI:10.1038/mt.2012.20
  • Peer Reviewed
• [Journal Article] Homo-catiomer integration into PEGylated polyplex micelle from block-catiomer for systemic antiangiogenic gene therapy for fibrotic pancreatic tumors. (2012)
  • Author(s): Q. Chen, K. O s ad a , T. Ishii, M. Ob
  • Journal Title: Biomaterials Volume: 33 Pages: 4722-4730
  • DOI: DOI:10.1016/j.biomaterials.2012.03.017
  • Peer Reviewed
• [Journal Article] Enhanced gene expression promoted by the quantized folding of pdna within polyplex micelles (2012)
  • Author(s): K. Osada, T. Shiotani, T. A. Tockary, D. Kobayashi, H. Oshima, S. Ikeda, R. J. Christie, K. Itaka, K. Kataoka
  • Journal Title: Biomaterials Volume: 33 Pages: 325-332
  • DOI: DOI:10.1016/j.biomaterials.2011.09.046
  • Peer Reviewed
• [Journal Article] Effect of polymer structure on micelles formed between siRNA and cationic block copolymer Comprising thiols and amidines (2011)
  • Author(s): R. J. Christie, K. Miyata, Y. Matsumoto, T. Nomoto, D. Menasco, T. -C. Lai, M. Pennisi, K. Osada, S. Fukushima, N. Nishiyama, Y. Yamasaki, K. Kataoka
  • Journal Title: Biomacromolecules Volume: 12 (9) Pages: 3174-3185
  • DOI: DOI: 10.1021/bm2006714
  • Peer Reviewed
• [Journal Article] Combination of chondroitin sulfate and polyplex micelles of poly(ethylene glycol)-poly{N'-[N-(2-aminoethyl)-2-aminoethyl]aspartamide} : A block copolymer for prolonged in vivo gene transfection with reducedtoxicity (2011)
  • Author(s): S. Uchida, K. Itaka, Q. Chen, K. Osada, K. Miyata, T. Ishii, M. Harada-Shiba, K. Kataoka
  • Journal Title: J. Control. Release Volume: 155 (2) Pages: 296-302
  • DOI: DOI: 10.1016/ j.jconrel.2011.04.026
  • Peer Reviewed
• [Journal Article] Polyplex micelles prepared from ω-cholesteryl PEG-polycation block copolymers for systemic gene delivery (2011)
  • Author(s): M. Oba, K. Miyata, K. Osada, R. J. Christie, M. Sanjoh, W. Li, S. Fukushima, T. Ishii, M. R. Kano, N. Nishiyama, H. Koyama, K. Kataoka
  • Journal Title: Biomaterials Volume: 32 (2) Pages: 652-663
  • DOI: DOI:10.1016/j.biomaterials.2010.09.022
  • Peer Reviewed
• [Journal Article] Quantized folding of plasmid DNA condensed with block catiomer into characteristic rod structures promoting transgene efficacy (2010)
  • Author(s): K. Osada, H. Oshima, D. Kobayashi, M. Doi, M. Enoki, Y. Yamasaki, K. Kataoka
  • Journal Title: J. Am. Chem. Soc. Volume: 132 (35) Pages: 12343-12348
  • DOI: DOI: 10.1021/ ja102739b
  • Peer Reviewed
• [Journal Article] Polyplex nanomicelle promotes hydrodynamic gene introduction to skeletal muscle (2010)
  • Author(s): K. Itaka, K. Osada, K. Morii, P. Kim, S. -H. Yun, K. Kataoka
  • Journal Title: J. Control. Release Volume: 143 (1) Pages: 112-119
  • DOI: DOI:10.1016/j.jconrel.2009.12.014
  • Peer Reviewed
• [Presentation] Control of DNAPackaging and Gene ExpressionEfficiency for Gene Delivery (2012)
  • Author(s): K. Osada, K. Kataoka
  • Organizer: 6thAnnual Symposium on Nanobiotechnology Kyoto Cell-Material Integration2012
  • Place of Presentation: KyotoUniversity, Kyoto, Japan
  • Year and Date: 20121107-1109
• [Presentation] Control of DNAPackaging and Gene ExpressionEfficiency for Gene Delivery (2012)
  • Author(s): K. Osada, K. Kataoka
  • Organizer: 1stInternational Conference on EmergingAdvanced Nanomaterials (ICEAN)
  • Place of Presentation: Mercure Hotel,Brisbane, Queensland, Australia
  • Year and Date: 20121107-1109
• [Presentation] Enhanced Gene Expression through theQuantized Folding of plasmid DNAwithin Polyplex Micelle (2012)
  • Author(s): K. Osada, K. Itaka, K. Kataoka,
  • Organizer: ACS Spring2012 National Meeting & Exposition
  • Place of Presentation: San Diego, USA
  • Year and Date: 20120325-0329
• [Presentation] Observation of PEG thickness ofPEG-PLys based polyplex micelle (2010)
  • Author(s): K. Osada, T. Tockary, K. Kataoka,
  • Organizer: International Chemical Congress ofPacific Basin Societies (Pacifichem2010)
  • Place of Presentation: Honolulu,USA)
  • Year and Date: 20101215-1220
• [Presentation] Quantized folding of plasmid DNApackaged into polyplex micelle. (2010)
  • Author(s): K. Osada, H. Oshima, D. Kobayashi, M.Doi, M. Enoki, Y, Yamasaki, K. Kataoka,
  • Place of Presentation: Zurich, Switzerland
  • Year and Date: 20100824-0827
• [Book] 高分子ミセル型ドラッグデリバリーシステム(DDS)-普及のインパクト ・ 課題と求められる新技術- (2013)
  • Author(s): 長田健介、片岡一則
  • Total Pages: 54-59
  • Publisher: 研究開発リーダー
• [Remarks]
  • URL: http://www.bmw.t.u-tokyo.ac.jp/member/osada.html
• [Patent(Industrial Property Rights)] 核酸デリバリー用ユニット構造体 (2012)
  • Inventor(s): 片岡一則、宮田完二郎、西山伸弘、 長田健介、渡辺秀美代
  • Industrial Property Rights Holder: 国立大学法人東京大学、ナノキャリ
  • Industrial Property Number: 発明 2012-102841
  • Filing Date: 2012-04-27
• [Patent(Industrial Property Rights)] 核酸送達用組成物及び担体組成物、そ (2011)
  • Inventor(s): 片岡一則、石井武彦、長田健介、陳
  • Industrial Property Rights Holder: 国立大学法人東京大学
  • Industrial Property Number: 発明 PCT/JP2011/065815
  • Filing Date: 2011-07-11
• [Patent(Industrial Property Rights)] ブロックコポリマー、ブロックコポリ (2010)
  • Inventor(s): 片岡一則、長田健介、岸村顕広、西
  • Industrial Property Rights Holder: 独立行政法人科学技術振興機構
  • Industrial Property Number: 発明 PCT/JP2010/058238
  • Filing Date: 2010-05-10