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2012 Fiscal Year Final Research Report

Kinetic-based analysis for quantized folding of closed-circular DNA

Research Project

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Project/Area Number 22750098
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Polymer chemistry
Research InstitutionThe University of Tokyo

Principal Investigator

OSADA Kensuke  東京大学, 大学院・工学系研究科, 准教授 (10396947)

Project Period (FY) 2010 – 2012
Keywords生体関連高分子 / DNA 凝縮
Research Abstract

Mechanistic analysis for structural formation of polyplex micelles as gene delivery system was demonstrated. Main object of this work was placed on understanding principle factors to determine the folding number of plasmid DNA (pDNA) within the polyplex micelles, where pDNA is packaged by highly regulated folding manner, namely quantized folding scheme. The research performed on a basis of kinetic analysis approved that folding process of pDNA is consistently described in the same way as kinetics of polymer crystallization. The deeper understanding on the structure formation of polyplex micelles enables secure molecular design for promoting effective gene delivery carriers.

  • Research Products

    (21 results)

All 2013 2012 2011 2010 Other

All Journal Article (11 results) (of which Peer Reviewed: 11 results) Presentation (5 results) Book (1 results) Remarks (1 results) Patent(Industrial Property Rights) (3 results)

  • [Journal Article] Bioactivepolymeric metallosomesself-assembled through blockcopolymer-metal complexation.2012

    • Author(s)
      K. Osada, H. Cabral, Y. Mochida, S. -ELee, K. Nagata, T. Matsuura, M.Yamamoto, Y. Anraku, A. Kishimura, N.Nishiyama, K. Kataoka
    • Journal Title

      J. Am.Chem. Soc.

      Volume: 134(32) Pages: 13172-13175

    • DOI

      DOI:10.1021/ja304615y

    • Peer Reviewed
  • [Journal Article] Effective transgene expression without toxicity by intraperitoneal administration of PEG-detachable polyplex micelles in mice with peritoneal dissemination.2012

    • Author(s)
      M. Kumagai, S. Shimoda, R. Wakabayashi, Y. Kunisawa, T. Ishii, K. O sa d a, K. Itaka, N. Nishiyama, K. Kataoka, K. Nakano
    • Journal Title

      J. Control. Release

      Volume: 160(3) Pages: 542-551

    • DOI

      DOI:10.1016/j.jconrel.2012.03.021

    • Peer Reviewed
  • [Journal Article] Targeted polymeric micelles for siRNA treatment of experimental cancer by intravenous injection.2012

    • Author(s)
      R. J. Christie, Y. Matsumoto, K.Miyata, T. Nomoto, S. Fukushima, K. O s a da , J. Halnaut, F. Pittella, H. -J. Kim, N. Nishiyama, K. Kataoka
    • Journal Title

      ACS Nano

      Volume: 6(6) Pages: 5174-5189

    • DOI

      DOI:10.1021/nn300942b

    • Peer Reviewed
  • [Journal Article] PEGylated polyplex with optimized PEG shielding enhances gene introduction in lungs by minimizing inflammatory responses2012

    • Author(s)
      S. Uchida, K. Itaka, Q. Chen, K . Os a da ,D. Kobayashi, H. Oshima, S. Ikeda, R.J. Christie, K. Itaka, K. Kataoka
    • Journal Title

      Mol Ther

      Volume: 20(6) Pages: 1196-1203

    • DOI

      DOI:10.1038/mt.2012.20

    • Peer Reviewed
  • [Journal Article] Homo-catiomer integration into PEGylated polyplex micelle from block-catiomer for systemic antiangiogenic gene therapy for fibrotic pancreatic tumors.2012

    • Author(s)
      Q. Chen, K. O s ad a , T. Ishii, M. Ob
    • Journal Title

      Biomaterials

      Volume: 33 Pages: 4722-4730

    • DOI

      DOI:10.1016/j.biomaterials.2012.03.017

    • Peer Reviewed
  • [Journal Article] Enhanced gene expression promoted by the quantized folding of pdna within polyplex micelles2012

    • Author(s)
      K. Osada, T. Shiotani, T. A. Tockary, D. Kobayashi, H. Oshima, S. Ikeda, R. J. Christie, K. Itaka, K. Kataoka
    • Journal Title

      Biomaterials

      Volume: 33 Pages: 325-332

    • DOI

      DOI:10.1016/j.biomaterials.2011.09.046

    • Peer Reviewed
  • [Journal Article] Effect of polymer structure on micelles formed between siRNA and cationic block copolymer Comprising thiols and amidines2011

    • Author(s)
      R. J. Christie, K. Miyata, Y. Matsumoto, T. Nomoto, D. Menasco, T. -C. Lai, M. Pennisi, K. Osada, S. Fukushima, N. Nishiyama, Y. Yamasaki, K. Kataoka
    • Journal Title

      Biomacromolecules

      Volume: 12 (9) Pages: 3174-3185

    • DOI

      DOI: 10.1021/bm2006714

    • Peer Reviewed
  • [Journal Article] Combination of chondroitin sulfate and polyplex micelles of poly(ethylene glycol)-poly{N'-[N-(2-aminoethyl)-2-aminoethyl]aspartamide} : A block copolymer for prolonged in vivo gene transfection with reducedtoxicity2011

    • Author(s)
      S. Uchida, K. Itaka, Q. Chen, K. Osada, K. Miyata, T. Ishii, M. Harada-Shiba, K. Kataoka
    • Journal Title

      J. Control. Release

      Volume: 155 (2) Pages: 296-302

    • DOI

      DOI: 10.1016/ j.jconrel.2011.04.026

    • Peer Reviewed
  • [Journal Article] Polyplex micelles prepared from ω-cholesteryl PEG-polycation block copolymers for systemic gene delivery2011

    • Author(s)
      M. Oba, K. Miyata, K. Osada, R. J. Christie, M. Sanjoh, W. Li, S. Fukushima, T. Ishii, M. R. Kano, N. Nishiyama, H. Koyama, K. Kataoka
    • Journal Title

      Biomaterials

      Volume: 32 (2) Pages: 652-663

    • DOI

      DOI:10.1016/j.biomaterials.2010.09.022

    • Peer Reviewed
  • [Journal Article] Quantized folding of plasmid DNA condensed with block catiomer into characteristic rod structures promoting transgene efficacy2010

    • Author(s)
      K. Osada, H. Oshima, D. Kobayashi, M. Doi, M. Enoki, Y. Yamasaki, K. Kataoka
    • Journal Title

      J. Am. Chem. Soc.

      Volume: 132 (35) Pages: 12343-12348

    • DOI

      DOI: 10.1021/ ja102739b

    • Peer Reviewed
  • [Journal Article] Polyplex nanomicelle promotes hydrodynamic gene introduction to skeletal muscle2010

    • Author(s)
      K. Itaka, K. Osada, K. Morii, P. Kim, S. -H. Yun, K. Kataoka
    • Journal Title

      J. Control. Release

      Volume: 143 (1) Pages: 112-119

    • DOI

      DOI:10.1016/j.jconrel.2009.12.014

    • Peer Reviewed
  • [Presentation] Control of DNAPackaging and Gene ExpressionEfficiency for Gene Delivery2012

    • Author(s)
      K. Osada, K. Kataoka
    • Organizer
      6thAnnual Symposium on Nanobiotechnology Kyoto Cell-Material Integration2012
    • Place of Presentation
      KyotoUniversity, Kyoto, Japan
    • Year and Date
      20121107-1109
  • [Presentation] Control of DNAPackaging and Gene ExpressionEfficiency for Gene Delivery2012

    • Author(s)
      K. Osada, K. Kataoka
    • Organizer
      1stInternational Conference on EmergingAdvanced Nanomaterials (ICEAN)
    • Place of Presentation
      Mercure Hotel,Brisbane, Queensland, Australia
    • Year and Date
      20121107-1109
  • [Presentation] Enhanced Gene Expression through theQuantized Folding of plasmid DNAwithin Polyplex Micelle2012

    • Author(s)
      K. Osada, K. Itaka, K. Kataoka,
    • Organizer
      ACS Spring2012 National Meeting & Exposition
    • Place of Presentation
      San Diego, USA
    • Year and Date
      20120325-0329
  • [Presentation] Observation of PEG thickness ofPEG-PLys based polyplex micelle2010

    • Author(s)
      K. Osada, T. Tockary, K. Kataoka,
    • Organizer
      International Chemical Congress ofPacific Basin Societies (Pacifichem2010)
    • Place of Presentation
      Honolulu,USA)
    • Year and Date
      20101215-1220
  • [Presentation] Quantized folding of plasmid DNApackaged into polyplex micelle.2010

    • Author(s)
      K. Osada, H. Oshima, D. Kobayashi, M.Doi, M. Enoki, Y, Yamasaki, K. Kataoka,
    • Place of Presentation
      Zurich, Switzerland
    • Year and Date
      20100824-0827
  • [Book] 高分子ミセル型ドラッグデリバリーシステム(DDS)-普及のインパクト ・ 課題と求められる新技術-2013

    • Author(s)
      長田健介、片岡一則
    • Total Pages
      54-59
    • Publisher
      研究開発リーダー
  • [Remarks]

    • URL

      http://www.bmw.t.u-tokyo.ac.jp/member/osada.html

  • [Patent(Industrial Property Rights)] 核酸デリバリー用ユニット構造体2012

    • Inventor(s)
      片岡一則、宮田完二郎、西山伸弘、 長田健介、渡辺秀美代
    • Industrial Property Rights Holder
      国立大学法人東京大学、ナノキャリ
    • Industrial Property Number
      発明 2012-102841
    • Filing Date
      2012-04-27
  • [Patent(Industrial Property Rights)] 核酸送達用組成物及び担体組成物、そ2011

    • Inventor(s)
      片岡一則、石井武彦、長田健介、陳
    • Industrial Property Rights Holder
      国立大学法人東京大学
    • Industrial Property Number
      発明 PCT/JP2011/065815
    • Filing Date
      2011-07-11
  • [Patent(Industrial Property Rights)] ブロックコポリマー、ブロックコポリ2010

    • Inventor(s)
      片岡一則、長田健介、岸村顕広、西
    • Industrial Property Rights Holder
      独立行政法人科学技術振興機構
    • Industrial Property Number
      発明 PCT/JP2010/058238
    • Filing Date
      2010-05-10

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Published: 2014-08-29  

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