2011 Fiscal Year Final Research Report
Development of highly-sensitive labeling agents by using "DNA dots"
| Project/Area Number |
22750149
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Chemistry related to living body
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| Research Institution | Nagoya University |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2011
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| Keywords | DNA / 電子移動 / インスレーター / シクロヘキサン / 蛍光 / 人工塩基対 / NMR / 量子収率 |
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| Research Abstract |
In this study, we aimed to develop highly-sensitive labeling agents by incorporating fluorophores into DNA. We developed artificial base pairs by incorporating cyclohexane derivatives between a fluorophore and nucleobases. As a result, quantum yields of fluorophores such as pyrene and perylenediimide were drastically enhanced. In addition, we demonstrated that the quantum yield of FITC was also improved by incorporating cyclohexane derivatives.
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