Functional elucidation of the AMP metabolic pathway in Archaea

2011 Fiscal Year Final Research Report

• Project/Area Number: 22750150
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Chemistry related to living body
• Research Institution: Kyoto University
• Principal Investigator: SATO Takaaki 京都大学, 大学院・工学研究科, 助教 (60571411)
• Project Period (FY): 2010 – 2011
• Keywords: アーキア / 微生物代謝 / ペントース代謝 / AMP / バイオテクノロジー

Research Abstract

In order to elucidate the function of an archaeal AMP metabolic pathway, biochemical and structural analyses of enzymes composing the pathway were performed. The results revealed that the AMP metabolic pathway is dramatically activated by AMP and ADP and degrades not only AMP but also CMP and UMP. These results suggested that the physiological functions of this pathway may be involved in the assimilation of extracellular nucleotides and/or in salvaging energy from AMP/CMP/UMP when intracellular energy levels are low, and AMP/ADP concentrations are relatively high.

Research Products

• [Journal Article] Dynamic, ligand-dependent conforma-tional change triggers the reaction of ribose-1, 5-bisphosphate isomerase from Thermococcus kodakarensis KOD1 (2012)
  • Author(s): Akira Nakamura, Masahiro Fujihashi, Riku Aono, Takaaki Sato, Yosuke Nishiba, Shosuke Yoshida, Ayumu Yano, Haruyuki Atomi, Tadayuki Imanaka, and Kunio Miki
  • Journal Title: J. Biol. Chem
  • DOI: DOI:10.1074/jbc.M112.349423
  • Peer Reviewed
• [Journal Article] Application of Hyperthermophiles and their Enzymes (2011)
  • Author(s): Haruyuki Atomi, Takaaki Sato and Tamotsu Kanai
  • Journal Title: Current Opinion in Biotechnology Volume: vol.22, No.5 Pages: 618-626
  • DOI: DOI:10.1016/j.copbio.2011.06.010
  • Peer Reviewed
• [Journal Article] Novel Metabolic Pathways in Archaea (2011)
  • Author(s): Takaaki Sato and Haruyuki Atomi
  • Journal Title: Current Opinion in Microbiology Volume: vol.14, No.3 Pages: 307-314
  • DOI: DOI:10.1016/j.mib.2011.04.014
  • Peer Reviewed
• [Presentation] Archaea特異的AMP代謝経路の生理的機能の解明 (2011)
  • Author(s): 佐藤喬章、青野陸、中村顕、西谷優一、藤橋雅宏、矢野歩、吉田昭介、今中忠行、三木邦夫、跡見晴幸
  • Organizer: 極限環境生物学会
  • Place of Presentation: 長崎大学坂本キャンパス1良順会館
  • Year and Date: 2011-11-28
• [Presentation] Novel enzymes involved in pentose metabolism in the Archaea (2011)
  • Author(s): Takaaki Sato, Riku Aono, Akira Nakamura, Yuichi Nishitani, Masahiro Fujihashi, Ayumu Yano, Shosuke Yoshida, Tadayuki Imanaka, Kunio Miki, and Haruyuki Atomi
  • Organizer: Japanese-German Workshop on Enzyme Technology
  • Place of Presentation: Toyama, Japan
  • Year and Date: 2011-09-14
• [Presentation] Unique metabolism involving archaeal Rubiscos (2011)
  • Author(s): Takaaki Sato
  • Organizer: Wageningen University and Research Centre/Kyoto University Joint Workshop
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2011-08-26
• [Presentation] 超好熱性アーキアThermococcus kodakarensisにおけるペントース代謝の解析 (2010)
  • Author(s): 佐藤喬章、今中忠行、跡見晴幸
  • Organizer: 極限環境生物学会
  • Place of Presentation: 京都大学宇治キャンパスおうばくプラザ
  • Year and Date: 2010-11-16
• [Presentation] Studies on a novel AMP metabolic pathway in Archaea (2010)
  • Author(s): Takaaki Sato, Ayumu Yano, Shosuke Yoshida, Tadayuki Imanaka, and Haruyuki Atomi
  • Organizer: 8th Extremophiles
  • Place of Presentation: Ponta Delgada, Azores, Portugal
  • Year and Date: 2010-09-14
• [Book] "Microbial inorganic carbon fixation" Encyclopedia of Life Sciences (2010)
  • Author(s): Takaaki Sato, and Haruyuki Atomi
  • Total Pages: 1-12
• [Book] "超好熱菌ゲノム情報に基づいた新規代謝酵素・経路の解明"酵素利用技術大系、第5編第5章第2節 (2010)
  • Author(s): 跡見晴幸、佐藤喬章、横大路裕介、今中忠行
  • Total Pages: 510-517