Development of the Artificial Oxidase Driven by Visible Light

2012 Fiscal Year Final Research Report

• Project/Area Number: 22750152
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Chemistry related to living body
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: TAINAKA Kazuki 独立行政法人理化学研究所, 合成生物学研究グループ, 研究員 (80506113)
• Project Period (FY): 2010 – 2012
• Keywords: 光アンテナ / Ru(II)錯体修飾DNA / 可視光 / 光電流応答 / ピロロキノリンキノン / グルコース脱水素酵素

Research Abstract

In this project, we developed a photoelectric transducer consist of Ru(II) complex as a light-harvesting antenna and DNA scaffold as a charge transporter. The DNA-modified films tethering Ru(II) complex showed cathodic photocurrent under visible light irradiation due to photoinitiated hole transport through DNA duplexes.

Research Products

• [Journal Article] Positional effects of phosphorylation on the stability and morphology of tau-related amyloid fibrils. (2012)
  • Author(s): Inoue M, Konno T, Tainaka K, Nakata E, Yoshida HO, Morii T.
  • Journal Title: Biochemistry Volume: 51 Pages: 1396-1406
  • DOI: doi:10.1021/bi201451z
  • Peer Reviewed
• [Journal Article] Generation of singlet oxygen during photosensitized one-electron oxidation of DNA. (2012)
  • Author(s): Osakada Y, Kawai K, Tachikawa T, Fujitsuka M, Tainaka K, Tero-Kubota S, Majima T.
  • Journal Title: Chemistry Volume: 18 Pages: 1060-1063
  • DOI: doi:10.1002/chem.201101964
  • Peer Reviewed
• [Journal Article] ICON probes: synthesis and DNA methylation typing. (2011)
  • Author(s): Tainaka K, Okamoto A.
  • Journal Title: Curr Protoc Nucleic Acid Chem. Volume: 8 Pages: Unit 8.7.1-17.
  • DOI: doi: 10.1002/0471142700.nc0807s47
  • Peer Reviewed
• [Journal Article] Sequence dependence of excess electron transfer in DNA. (2010)
  • Author(s): Tainaka K, Fujitsuka M, Takada T, Kawai K, Majima T.
  • Journal Title: J Phys Chem B. Volume: 114 Pages: 14657-14663
  • DOI: doi:10.1021/jp1024685
  • Peer Reviewed
• [Journal Article] Design strategies of fluorescent biosensors based on biological macromolecular receptors. (2010)
  • Author(s): Tainaka K, Sakaguchi R, Hayashi H, Nakano S, Liew FF, Morii T.
  • Journal Title: Sensors (Basel). Volume: 10 Pages: 1355-1376
  • DOI: doi:10.3390/s100201355
  • Peer Reviewed
• [Journal Article] An in vivo fluorescent sensor reveals intracellular ins(1,3,4,5)P4 dynamics in single cells. (2010)
  • Author(s): Sakaguchi R, Tainaka K, Shimada N, Nakano S, Inoue M, Kiyonaka S, Mori Y, Morii T.
  • Journal Title: Angew Chem Int Ed Engl. Volume: 49 Pages: 2150-2153
  • DOI: doi:10.1002/anie.200903951
  • Peer Reviewed
• [Patent(Industrial Property Rights)] 非天然型塩基を含むオリゴデオキシヌクレオチドの切断による5'末端リン酸化オリゴデオキシヌクレオチドの製造方法 (2012)
  • Inventor(s): 上田泰己、池田修司、田井中一貴
  • Industrial Property Rights Holder: 同上
  • Industrial Property Number: 特願,2012-084249
  • Filing Date: 2012-04-02
• [Patent(Industrial Property Rights)] 非天然型塩基を含むオリゴデオキシヌクレオチドの切断による5'末端リン酸化オリゴデオキシヌクレオチドの製造方法 (2012)
  • Inventor(s): 上田泰己、池田修司、田井中一貴
  • Industrial Property Rights Holder: 同上
  • Industrial Property Number: PCT,JP2013/058349
  • Filing Date: 2012-04-02
  • Overseas