2012 Fiscal Year Final Research Report
Development of a method for regulating transcription by light sensitive hairpin-type peptide nucleic acid
| Project/Area Number |
22750155
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Chemistry related to living body
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| Research Institution | Osaka University |
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Principal Investigator |
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| Research Collaborator |
KATO Nobuo 大阪大学, 産業科学研究所, 教授 (50150537)
SAWADA Shinjiro 大阪大学, 大学院・理学研究科, 博士後期課程
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| Project Period (FY) |
2010 – 2012
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| Keywords | 転写制御 / バイオテクノロジー / 核酸 / 遺伝子 / 有機化学 |
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| Research Abstract |
We synthesized a hairpin-type peptide nucleic acid-AZO(hairpin-type PNA-AZO) that tethers a Watson-Crick strand and a Hoogsteen strand with visible light-sensitive azobenzene linkers. We confirmed the azobenzene linkers enhanced the affinity to its complementary DNA sequence and afforded a 1;1 complex formation with its target DNA sequence. Visible light irradiation to the hairpin-type PNA-AZO altered its binding property to the target DNA and thetrans-isoform exhibited higher binding affinity compared to the cis-isoform. We further examined if hairpin-type-PNA-AZO can regulate transcription of plasmid encoding hemagglutinine(HA) protein of influenza A/H1N1 virus and green fluorescence protein(GFP) of Aequorea victoria in HeLa cell by fluorescence microscopy. As a result, the trans-form of hairpin-type PNA-AZO reduced the expression of HA-GFP protein in cells, while the cis-form didn’t interfer the expression. From these results, we can regulatetranscription of DNA in cells by controlling the configuration of azobenzene unit of hairpin-type PNA-AZO via visible light irradiation.
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