2011 Fiscal Year Final Research Report
Economical and Efficient Production of Cardiac Myocytes Using Small Molecule-Responsive Artificial Receptors For Wnt3a Signal Transduction
| Project/Area Number |
22760607
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Kyoto University |
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Principal Investigator |
SOGO Takahiro 京都大学, 生命科学系・キャリアパス形成ユニット, 研究員 (30561972)
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| Project Period (FY) |
2010 – 2011
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| Keywords | 心筋再生 / 幹細胞 / シグナル伝達 / 受容体 / 抗体 / Wnt3a |
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| Research Abstract |
We constructed chimeric receptors in which single-chain Fv of anti-fluorescein(FL) antibody was tethered to transmembrane/cytoplasmic domains of Frizzled8 and LRP6 that are receptors for Wnt3a. Mouse ES cells or iPS cells transduced with these chimeric receptors could activate Wnt3a signaling in response to FL-conjugated BSA(BSA-FL) as a cognate ligand, and could efficiently differentiate into cardiomyocyte when they are stimulated with BSA-FL, in the absence of Wnt3a.
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