2013 Fiscal Year Final Research Report
Development of value-added chemicals from light using light-driven vesicles and cell factory.
| Project/Area Number |
22760608
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Biofunction/Bioprocess
|
|---|
| Research Institution | Kobe University |
|---|
Principal Investigator |
HARA Kiyotaka 神戸大学, 自然科学系先端融合研究環, 准教授 (40434378)
|
|---|
| Project Period (FY) |
2010-04-01 – 2014-03-31
|
| Keywords | ATP / ATP synthase / Membrane vesicle / Photoenergetics / Rhodopsin / Vesicles |
|---|
| Research Abstract |
We prepared ATP photosynthetic vesicles from inside-out membranes of Escherichia coli cells that express delta-rhodopsin (a novel light-driven H+ transporter) and TFoF1-ATP synthase (a thermostable ATP synthase). These vesicles showed lightdependent ATP synthesis. Furthermore, coupling the ATP photosynthetic vesicles with an ATP-hydrolyzing hexokinase enabled light-dependent glucose consumption. The ATP photosynthetic vesicles indicate their potential to applied to light-driven ATPregenerating bioprocess for various ATP-hydrolyzing bioproductions.
|
