2011 Fiscal Year Final Research Report
Development of efficient peptide drug discovery system by using highly specific tag-mediated protein immobilization technique
| Project/Area Number |
22760609
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Okayama University |
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Principal Investigator |
IMANAKA Hiroyuki 岡山大学, 大学院・自然科学研究科, 助教 (10379711)
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| Project Period (FY) |
2010 – 2011
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| Keywords | ペプチド / タンパク質 / 固定化 / 相互作用 / 創薬 / スクリーニング / ランダムライブラリー / 分子標的薬剤 |
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| Research Abstract |
We have tried to develop an efficient peptide drug discovery system utilizing highly specific tag-mediated protein immobilization technique. Two cancer-related proteins, FOXP3 and NFkB(p50), were adopted as targets and site specific inhibitory peptides were screened from T7 phage random peptide library. The clear correlation between effective isolation of candidate peptides and pretreatment of phage library with solid substrate for protein immobilization was found. In addition, a number of functional inhibitory peptides with high binding affinity with target proteins were successfully obtained.
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