2011 Fiscal Year Final Research Report
Cell-free synthesis and in vitro reconstitution of cellulase/hemicellulases supramolecular complex
| Project/Area Number |
22760612
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Nihon University |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2011
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| Keywords | 生物機能工学 |
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| Research Abstract |
Cellulase/hemicellulases supramolecular complex(cellulosome) from thermophilic anaerobe Clostridium thermocellum shows a high saccharification activity for crystalline cellulose. In this study, we analyzed the saccharification activity of the C. thermocellum supramolecular cellulosome, which is in vitro reconstituted from cell-free synthesized cellulosomal components. As a result, it was revealed that the saccharification activity for crystalline cellulose increased along with an increase in the number of cellulosome-binding domains(cohesins) in the scaffolding protein. Furthermore, we successfully isolated four cohesin genes from a compost metagenome.
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