2011 Fiscal Year Final Research Report
A buildup approach to the design of a small-sized protein via an autonomously-folded short segment
| Project/Area Number |
22760617
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | National Institute of Advanced Industrial Science and Technology |
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Principal Investigator |
WATANABE Hideki 独立行政法人産業技術総合研究所, バイオメディカル研究部門, 研究員 (90422089)
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| Project Period (FY) |
2010 – 2011
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| Keywords | 生物機能工学 / 人工蛋白質 / 分子進化工学 / 抗体医薬 |
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| Research Abstract |
This study established a methodology for the design of small-sized proteins on the basis of segment elongation by using chignolin that was introduced as a structural support. Using T7 phage displayed library, we selected artificial proteins with affinity for antibody Fc region that was used as a model target. Function and three dimensional structures of artificial proteins were analyzed in detail by using surface plasmon resonance, NMR, and X-ray crystallography.
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Research Products
(7 results)
