Mechanism to establish cohesion specifically between sister chromatids

2011 Fiscal Year Final Research Report

• Project/Area Number: 22770005
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Genetics/Genome dynamics
• Research Institution: The University of Tokyo
• Principal Investigator: TAKASHI Sutani 東京大学, 分子細胞生物学研究所, 助教 (30401524)
• Project Period (FY): 2010 – 2011
• Keywords: ゲノム構築 / 再編 / 維持

Research Abstract

Sister chromatids, products of DNA replication, are held together by cohesin complex, which is vital for faithful chromosome segregation. When the cohesion is established, replication-dependent acetylation of a cohesin subunit Smc3 by Eco1 acetylase plays a critical role. In this study, I investigated how the cohesin acetylation is regulated in the cell cycle and how Eco1's function is coupled with DNA replication. Using an antibody specifically recognizing acetylated Smc3, I found the following.(1) Cohesin is deacetylated specifically by Hos1. This deacetylation is important in recycling cohesion complex for the next cell cycle.(2) Efficient acetylation of cohesin in S phase requires the presence of Ctf18-RFC, Ctf4 and Chl1, all of which are implicated in DNA replication. Subsequent analyses suggested that coordinated replication machinery action, particularly lagging strand synthesis, may play an important role in coupling of cohesin acetylation to DNA replication

Research Products

• [Journal Article] An Smc3 acetylation cycle is essential for establishment of sister chromatid cohesion (2010)
  • Author(s): Beckouet F, Hu B, Roig MB, Sutani T, Komata M, Uluocak P, Katis VL, Shirahige K, Nasmyth K
  • Journal Title: Mol. Cell Volume: vol.39 Pages: 689-699
  • Peer Reviewed
• [Journal Article] The RSC chromatin-remodeling complex influences mitotic exit and adaptation to the spindle assembly checkpoint by controlling the Cdc14 phosphatase (2010)
  • Author(s): Rossio V, Galati E, Ferrari M, Pellicioli A, Sutani T, Shirahige K, Lucchini G, Piatti S
  • Journal Title: J. Cell. Biol Volume: vol.191 Pages: 981-997
  • Peer Reviewed
• [Presentation] 姉妹染色分体間の接着確立に必須なSmc3アセチル化の細胞周期制御 (2011)
  • Author(s): 須谷尚史、古俣麻希子、鶴田祥悠、九十九雅理、白髭克彦
  • Organizer: 第28回染色体ワークショップ
  • Place of Presentation: 石川県加賀市
  • Year and Date: 2011-01-12
• [Presentation] Cell cycle regulation of Smc3 acetylation required for sister chromatid cohesion establishment (2010)
  • Author(s): Sutani T, Komata M, Tsuruta T, Tsukumo M, Shirahige K
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 兵庫県神戸市
  • Year and Date: 2010-12-09