2011 Fiscal Year Final Research Report
Regulation of DNA methylation at SINEs during mouse male germ-cell development
Project/Area Number |
22770009
|
Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Single-year Grants |
Research Field |
Genetics/Genome dynamics
|
Research Institution | Kyushu University |
Principal Investigator |
|
Project Period (FY) |
2010 – 2011
|
Keywords | ゲノム機能・発現 / エピジェネティック制御 |
Research Abstract |
To elucidate regulation of SINE retrotransposons during mammalian germ-cell development, DNA methylation states of mouse B1 and B2 SINE were studied. This study revealed(1) SINEs become demethylated in primordial germ cells, then re-methylated in fatal prospermatogonia,(2) after birth, they keep methylated during spermatogenesis,(3) SINE copies neighboring transcription start sites are involved in regulation of the gene expression,(4) the germ cell-specific small RNAs(piRNAs) are dispensable for SINE methylation and regulation, and(5) SINE methylation depends on Dnmt3a with varied dependencies according to their genomic and nuclear locations. Thus, the regulation of SINE methylation differs significantly from those of LINEs and endogenous retroviruses.
|
Research Products
(8 results)
-
-
[Journal Article] Locus-and domain-dependent control of DNA methylation at mouse B1 retrotransposons during male germ cell development2011
Author(s)
Ichiyanagi, K., Li, Y., Watanabe, T., Ichiyanagi, T., Fukuda, K., Kitayama, J., Yamamoto, Y., Kuramochi-Miyagawa, S., Nakano, T., Yabuta, Y., Seki, Y., Saitou, M., and Sasaki, H
-
Journal Title
Genome Res
Volume: 21
Pages: 2058-2066
-
[Journal Article] Role for piRNAs and non-coding RNA in de novo DNA methylation of2011
Author(s)
Watanabe, T., Tomizawa, S., Mitsuya, K., Totoki, Y., Yamamoto, Y., Kuramochi-Miyagawa, S., Iida, N., Hoki, Y., Murphy, P. J., Toyoda, A., Gotoh, K., Hiura, H., Arima, T., Fujiyama, A., Sado, T., Shibata, T., Nakano, T., Lin, H., Ichiyanagi, K., Soloway, P.D. and Sasaki, H.
-
Journal Title
the imprinted mouse Rasgrf1 locusthe imprinted mouse Rasgrf1 locusthe imprinted mouse Rasgrf1 locus
Volume: 332
Pages: 848-852
-
-
[Presentation] Locusand domain-dependent control of DNA methylation at mouse B1 retrotransposon during male germ cell development2012
Author(s)
2) Ichiyanagi, K., Li, Y., Watanabe, T., Fukuda, K., Kitayama, J., Seki, Y., Yabuta, Y., Yamamoto, Y., Ichiyanagi, T., and Sasaki H.
Organizer
Gordon Research Conference
Place of Presentation
MA, U. S. A
Year and Date
20120000
-
-
-