Analysis of the X chromosome inactivation-reactivation cycle throughout the mouse life cycle

2011 Fiscal Year Final Research Report

• Project/Area Number: 22770010
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Genetics/Genome dynamics
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: SUGIMOTO Michihiko 独立行政法人理化学研究所, 動物変異動態解析技術開発チーム, 開発研究員 (10373317)
• Project Period (FY): 2010 – 2011
• Keywords: X染色体不活性化 / マウス / 初期胚発生 / エピジェネティクス

Research Abstract

It is known that the X chromosome of female mammals shows inactivation-reactivation cycles throughout the life cycle, especially at the peri-implantation stage, the changes of the X chromosome activity occur during short period. In this study, it was indicated that the re-inactivation of the X chromosome in the epiblast, the future embryo proper, occurs just after implantation. Furthermore, the dynamics of the DNA methylation of the inactive X linked genes during the X reactivation in primordial germ cells was elucidated.

Research Products

• [Journal Article] RNAi mediated knockdown of Xist can rescue the impaired postimplantation development of cloned mouse embryos (2011)
  • Author(s): Matoba S., Inoue K., Kohda T., Sugimoto M., Mizutani E., Ogonuki N., Nakamura T., Abe K., Nakano T., Ishino F. and Ogura A.
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: 108(51) Pages: 20621-20626
  • Peer Reviewed
• [Journal Article] Impeding Xist expression from the active X chromosome improves mouse somatic cell nuclear transfer (2010)
  • Author(s): Inoue K., Kohda T., Sugimoto M., Sado T., Ogonuki N., Matoba S., Shiura H., Ikeda R., Mochida K., Fujii T., Sawai K., Otte A. P., Tian C., Yang X., Ishino F., Abe K. and Ogura A.
  • Journal Title: Science Volume: 330 Pages: 496-499
  • Peer Reviewed
• [Presentation] t^<w5>劣性致死変異の責任遺伝子Vps52の機能解析/Functional analysis of Vps52, a responsible gene for the t^<w5> recessive lethal mutation (2011)
  • Author(s): 杉本道彦、近藤昌代、廣瀬美智子、小倉淳郎、阿部訓也
  • Organizer: 日本遺伝学会83回大会
  • Place of Presentation: 京都
  • Year and Date: 20110920-22
• [Presentation] 特異な進化履歴を持つマウスt-コンプレックスのゲノム構造解析/Unexplored mouse genome : structure and evolution of the t-complex (2011)
  • Author(s): 阿部訓也、沼田興治、池田理恵子、杉本道彦、太田聡史
  • Organizer: 日本遺伝学会83回大会
  • Place of Presentation: 京都
  • Year and Date: 20110920-22
• [Presentation] Dynamics of epigenomic changes during development of early embryos and primordial germ cells in mice (2011)
  • Author(s): Kuniya Abe, Koji Numata, Rieko Ikeda, Hirosuke Shiura and Michihiko Sugimoto
  • Organizer: EMBO Workshop "50 Years of X-inactivation Research"
  • Place of Presentation: Oxford
  • Year and Date: 20110720-24
• [Presentation] マウスt-complex劣性致死変異の同定により見えてきたVps52の機能-哺乳類初期発生における多能性細胞の分化制御因子としての役割- (2010)
  • Author(s): 杉本道彦、阿部訓也
  • Organizer: 日本遺伝学会82回大会ワークショップ「マウス・ラットをモデルとした哺乳類遺伝学」
  • Place of Presentation: 札幌(招待講演)
  • Year and Date: 20100920-22
• [Remarks]
  • URL: http://www.brc.riken.jp/lab/intro_heni.html