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2012 Fiscal Year Final Research Report

A metabolic pathway for generation of GDP-glucose in higher plants

Research Project

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Project/Area Number 22770030
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Plant molecular biology/Plant physiology
Research InstitutionSaitama University

Principal Investigator

KOTAKE Toshihisa  埼玉大学, 大学院・理工学研究科, 准教授 (20334146)

Project Period (FY) 2010 – 2012
Keywords細胞壁 / 糖ヌクレオチド / グルコマンナン / GDP-マンノース / GDP-グルコース
Research Abstract

The konjac(kjc)1 mutation appeared to affect glucomannan content. Recombinant KJC1 and KJC2 expressed in E. coli did not have any enzymatic activity, but they stimulated GDP-mannose/GDP-glucose synthase activity of VTC1. The results suggest that KJC1 and 2 are involved in glucomannan synthesis through the regulation of the synthesis of GDP-mannose and GDP-glucose catalyzed by VTC1.

  • Research Products

    (7 results)

All 2013 2012 2011 2010

All Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (3 results) Book (1 results)

  • [Journal Article] β-Galactosyl Yariv reagent binds to the β-1,3-galactan of arabinogalactan-proteins2013

    • Author(s)
      Kitazawa K., Tryfona T., Yoshimi Y., Hayashi Y., Kawauchi S., Antonov L., Tanaka H., Takahashi T., Kaneko S., Dupree P., Tsumuraya Y. and Kotake T
    • Journal Title

      Plant Physiol.

      Volume: 161 Pages: 1117-1126

    • DOI

      doi:10.1104/pp.112.211722.

    • Peer Reviewed
  • [Journal Article] Structural characterisation of Arabidopsis leaf arabinogalactan polysaccharides2012

    • Author(s)
      Tryfona T., Liang H.-C., Kotake T., Tsumuraya Y., Stephens E. and Dupree P
    • Journal Title

      Plant Physiol

      Volume: 160 Pages: 653-666

    • DOI

      doi:10.1104/pp.112.202309.

    • Peer Reviewed
  • [Journal Article] Generation of nucleotide sugars for biomass formation in plants2010

    • Author(s)
      Kotake T., Hirosawa C., Ando Y. and Tsumuraya Y
    • Journal Title

      Plant Biotechnol

      Volume: 27 Pages: 231-236

    • Peer Reviewed
  • [Presentation] KONJAC1 and 2 Are Genes Involved in Glucomannan and L-Ascorbate Synthesis in Arabidopsis2012

    • Author(s)
      Toshihisa Kotake, Noriaki Tajima, Jenny Mortimer, Shota Sawake, Xiaolan Yu, Paul Dupree, and Yoichi Tsumuraya
    • Organizer
      International Conference for Arabidopsis Research
    • Place of Presentation
      オーストリア・ウィーン
    • Year and Date
      2012-07-03
  • [Presentation] 細胞壁グルコマンナン合成におけるKONJAC1, 2の役割2012

    • Author(s)
      小竹敬久、Jenny Mortimer, 田島範明、宮崎祐一、Xiaolan Yu, Paul Dupree, 円谷陽一
    • Organizer
      日本植物生理学会
    • Place of Presentation
      京都府京都市・京都産業大学
    • Year and Date
      2012-03-17
  • [Presentation] KONJAC1 and 2 are genes involved in glucomannan and vitamin C synthesis in Arabidopsis2011

    • Author(s)
      Toshihisa Kotake, Noriaki Tajima, Yuichi Miyazaki, Jenny Mortimer, Xiaolan Yu, Paul Dupree, Yoichi Tsumuraya
    • Organizer
      The 4th Conference on Biosynthesis of Plant Cell Wall
    • Place of Presentation
      兵庫県淡路市・淡路夢舞台
    • Year and Date
      2011-10-06
  • [Book] 植物細胞壁(西谷和彦・梅澤俊明編)2013

    • Author(s)
      小竹敬久(部分執筆)
    • Total Pages
      349、14-18、62-65、101-102、314-317
    • Publisher
      講談社

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Published: 2014-08-29   Modified: 2015-09-28  

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