2012 Fiscal Year Final Research Report
Molecular mechanisms of the activation of the nitrogen transporter in cyanobacteria
| Project/Area Number |
22770034
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | Nagoya University |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2012
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| Keywords | ラン藻 / 輸送体 / 活性制御 |
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| Research Abstract |
We analyzed the molecular mechanisms of the regulatory systems of three kinds ofnitrogen transporters (LtnT, CynABD, FocA) in cyanobacteria. We identified a novel protein (LtnE) that is involved in the activation of the LtnT transporter in a freshwater cyanobacterium. We clarified that the PII protein, which was a regulatory protein of various nitrogen assimilation proteins, was involved in the regulation of the activity of the CynABD transporter. We clarified that the C-terminal region of the FocA transporter inhibited the transport activity of the transporter and the FocA transport activity could be inactivated in the presence of ammonium in the medium. In addition, we established the culture method of a marine cyanobacterium and clarified that there was an unknownnitrite transport mechanism in the marine cyanobacterium.
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