2011 Fiscal Year Final Research Report
Analyses of functional RNAs and target genes involves in leg regeneration using transgenic cricket, Gryllus bimaculatus
| Project/Area Number |
22770057
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Morphology/Structure
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| Research Institution | The University of Tokushima |
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Principal Investigator |
NAKAMURA Taro 徳島大学, 産学官連携推進部, 講師 (80548834)
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| Project Period (FY) |
2010 – 2011
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| Keywords | 形態形成 / 組織再生 / 機能性RNA / 形質転換技術 |
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| Research Abstract |
We cloned several miRNAs from libraries of embryonic and regenerating tissues and observed these expressions in the embryos. We revealed expression patterns of cloned miRNAs. We found miR-8, which is a target of Wnt component gene in our miRNAs libraries. Moreover, to analyze the function of miRNA and its target genes, we established cell trajectory analysis using the transgenic cricket. Furthermore, we established gene-targeting systems using zinc finger nucleases(ZFNs) and transcription activator-like(TAL) effector nucleases(TALENs). Using these systems, we are now trying to investigate the function of the miRNA involves in the leg regeneration in cricket.
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[Journal Article] Functional analysis of the role of eyes absent and sine oculis in the developing eye of the cricket Gryllus bimaculatus2012
Author(s)
Takagi, A., Kurita, K., Terasawa, T., Nakamura, T., Bando, T., Moriyama, Y., Mito, T., Noji, S., and Ohuchi, H.
Volume
54
Pages
227-240
DOI
Peer Reviewed
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[Journal Article] Ancestral functions of Delta/ Notch signaling in the formation of body and leg segments in the cricket Gryllus bimaculatus2011
Author(s)
Mito, T., Shinmyo, Y., Kurita, K., Nakamura, T., Ohuchi, H., and Noji, S
Volume
138
Pages
3823-3833
DOI
Peer Reviewed
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[Journal Article] Cricket body size is altered by systemic RNAi against insulin signaling components and epidermal growth factor receptor2011
Author(s)
Dabour, N., Bando, T., Nakamura, T., Miyawaki, K., Mito, T., Ohuchi, H., and Noji, S
Volume
53
Pages
857-869
DOI
Peer Reviewed
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[Journal Article] Lowfat, a mammalian Lix1 homologue, regulates leg size and growth under the Dachsous/ Fat signaling pathway during tissue regeneration2011
Author(s)
Bando, T., Hamada, Y., Kurita, K., Nakamura, T., Mito, T., Ohuchi, H., and Noji, S
Volume
240
Pages
1440-1453
DOI
Peer Reviewed
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[Journal Article] Imaging of transgenic cricket embryos reveals cell movements consistent with a syncytial patterning mechanism2010
Author(s)
Nakamura, T., Yoshizaki, M., Ogawa, S., Okamoto, H., Shinmyo, Y., Bando, T., Ohuchi, H., Noji, S., and Mito, T.
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Journal Title
Curr. Biol
Volume: 20
Pages: 1641-1647
DOI
Peer Reviewed
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