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2012 Fiscal Year Final Research Report

Analyses of segregation of chloroplast nucleoids

Research Project

  • PDF
Project/Area Number 22770061
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Morphology/Structure
Research InstitutionNational Institute of Genetics (2011-2012)
The Institute of Physical and Chemical Research (2010)

Principal Investigator

KABEYA Yukihiro  国立遺伝学研究所, 新分野創造センター, 特任研究員 (20462674)

Project Period (FY) 2010 – 2012
Keywords葉緑体 / 核様体 / DNA 分配 / DNA 複製
Research Abstract

In this study, 41 proteins were identified as chloroplast nucleoid-associated proteins by LC-MS/MS analyses. And then, I analyzed about two high content unknown function proteins, cpNUC1 and cpNUC3. I found that (1) cpNUC1 protein has an ability of DNA compaction in vitro but is uninvolved in the cpDNA segregation, and that (2) cpNUC3 is involved in hidden break of rrn23 RNA but is uninvolved in the cpDNA segregation. In addition, I revealed that chloroplast DNA replication is regulated by the redox rather than chloroplast division/cell cycle

  • Research Products

    (9 results)

All 2013 2012 2011 2010

All Journal Article (6 results) (of which Peer Reviewed: 6 results) Presentation (3 results)

  • [Journal Article] Chloroplast DNA replication is regulated by the redox state independently of chloroplast division in Chlamydomonas reinhardtii2013

    • Author(s)
      Kabeya, Y. and Miyagishima, S
    • Journal Title

      Plant Physiol

      Pages: 2102-2112

    • Peer Reviewed
  • [Journal Article] Evidence for tubular mating structures induced in each mating type of heterothallic GONIUM PECTORALE (Volvocales, Chlorophyta)2012

    • Author(s)
      Mogi, Y., Hamaji, T., Suzuki, M., Ferris, P., Mori,T., Kabeya, Y., Miyagishima,S., and Nozaki, H
    • Journal Title

      J.Phycol

      Volume: 48 Pages: 670-674

    • Peer Reviewed
  • [Journal Article] Expression of the nucleus-encoded chloroplast division genes and proteins regulated by the algal cell cycle2012

    • Author(s)
      Miyagishima,S., Suzuki, K., Okazaki, K., and Kabeya, Y
    • Journal Title

      Mol.Biol.Evol

      Volume: 29 Pages: 2957-2970

    • Peer Reviewed
  • [Journal Article] Structure, regulation, and evolution of the plastid division machinery2011

    • Author(s)
      Miyagishima, S., Nakanishi, H., and Kabeya, Y
    • Journal Title

      Int.Rev.Cell.Mol.Biol

      Volume: 291 Pages: 115-153

    • Peer Reviewed
  • [Journal Article] Chloroplast division: squeezing the photosynthetic captive. Curr2010

    • Author(s)
      Miyagishima, S. and Kabeya, Y
    • Journal Title

      Opin.Microbiol

      Volume: 13 Pages: 738-746

    • Peer Reviewed
  • [Journal Article] The YlmG protein has a conserved function related to the distribution of nucleoids in chloroplasts and cyanobacteria2010

    • Author(s)
      Kabeya, Nakanishi, H., Suzuki, K., Ichikawa, T., Kondou, Matsui, M., and Miyagishima, S
    • Journal Title

      BMC Plant Biol

      Volume: 10 Pages: 57-69

    • Peer Reviewed
  • [Presentation] 葉緑体DNA 複製のレドックス制御2013

    • Author(s)
      壁谷如洋、宮城島進也
    • Organizer
      第54回日本植物生理学会年会
    • Place of Presentation
      岡山大
    • Year and Date
      2013-03-22
  • [Presentation] 葉緑体DNA 複製制御の解析2012

    • Author(s)
      壁谷如洋、宮城島進也
    • Organizer
      第53回日本植物生理学会年会
    • Place of Presentation
      京都産業大
    • Year and Date
      2012-03-17
  • [Presentation] 葉緑体プロテオームを基 盤とした逆遺伝学的解析および質量分析による葉緑体核様体タンパク質の同定2010

    • Author(s)
      壁谷如洋、鈴木健二、宮城島進也
    • Organizer
      第51回日本植物生理学会年会
    • Place of Presentation
      熊本大
    • Year and Date
      2010-03-18

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Published: 2014-08-29  

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