Exploring parasitic protists in animals inhabiting deep-sea methane seeps

2012 Fiscal Year Final Research Report

• Project/Area Number: 22770091
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Biodiversity/Systematics
• Research Institution: Japan Agency for Marine-Earth Science and Technology
• Principal Investigator: TAKISHITA Kiyotaka 独立行政法人海洋研究開発機構, 海洋・極限環境生物圏領域, 主任研究員 (90392951)
• Project Period (FY): 2010 – 2012
• Keywords: 深海 / 原生生物 / 寄生 / 進化 / 多様性

Research Abstract

Utilizing PCR techniques, the SSU rRNA gene from a novel alveolate was detected from gill tissues of the bivalves alyptogena spp. And Bathymodiolus spp. All of which harbor chemosynthetic bacteria and dominate methane seeps in Sagami Bay. In addition, the protist detected with PCR was localized within bacteriocytes of the bivalves with whole-mount fluorescence in situ hybridization.

Research Products

• [Journal Article] A novel alveolate in bivalves with chemosynthetic bacteria inhabiting deep-sea methane seeps (2013)
  • Author(s): Fumiya Noguchi, Masaru Kawato, Takao Yoshida, Yoshihiro Fujiwara, Katsunori Fujikura, Kiyotaka Takishita
  • Journal Title: Journal of Eukaryotic Microbiology Volume: Vol.60 Pages: 158-165
  • DOI: doi:10.1111/jeu.12017
  • Peer Reviewed
• [Journal Article] Lateral transfer of tetrahymanol-synthesizing genes has allowed multiple diverse eukaryote lineages to independently adapt to environments without oxygen (2012)
  • Author(s): Kiyotaka Takishita, Yoshito Chikaraishi, Michelle M. Leger, Eunsoo, Kim, Akinori Yabuki, Naohiko Ohkouchi, Andrew J. Roger
  • Journal Title: Biology Direct Volume: Vol.7 Pages: 5
  • DOI: doi:10.1186/1745-6150-7-5
  • Peer Reviewed
• [Journal Article] Multigene phylogenies of diverse Carpediemonas-like organisms identify the closest relatives of 'amitochondriate' diplomonads and retortamonads (2012)
  • Author(s): Kiyotaka Takishita, Martin Kolisko, Hiroshi Komatsuzaki, Akinori Yabuki, Yuji Inagaki, Ivan Cepicka, Pavla Smejkalova, Jeffrey D. Silberman, Tetsuo Hashimoto, Andrew J. Roger, Alastair G.B. Simpson
  • Journal Title: Protist Volume: Vol.163 Pages: 344-355
  • DOI: doi:10.1016/j.protis.2011.12.007
  • Peer Reviewed
• [Journal Article] Evolution of elongation factor-like (EFL) protein in Rhizaria is revised by radiolarian EFL gene sequences (2012)
  • Author(s): Yoshiyuki Ishitani, Ryoma Kamikawa, Akinori Yabuki, Masashi Tsuchiya, Yuji Inagaki, Kiyotaka Takishita
  • Journal Title: Journal of Eukaryotic Microbiology Volume: Vol.59 Pages: 367-373
  • DOI: doi:10.1111/j.1550-7408.2012.00626.x
  • Peer Reviewed
• [Journal Article] Algivore or phototroph? Plakobranchus ocellatus (Gastropoda) continuously acquires kleptoplasts and nutrition from multiple algal species in nature (2012)
  • Author(s): Taro Maeda, Euichi Hirose, Yoshito Chikaraishi, Masaru Kawato, Kiyotaka Takishita, Takao Yoshida, Heroen Verbruggen, Jiro Tanaka, Shigeru Shimamura, Yoshihiro Takaki, Masashi Tsuchiya, Kenji Iwai, Tadashi Maruyama
  • Journal Title: PLoS ONE Volume: Vol.7 Pages: e42024
  • DOI: doi:10.1371/journal.pone.0042024
  • Peer Reviewed
• [Journal Article] Multigene phylogenetic analyses including diverse radiolarian species support the "Retaria" hypothesis - the sister relationship of Radiolaria and Foraminifera (2011)
  • Author(s): Yoshiyuki Ishitani, Sohta Ishikawa, Yuji Inagaki, Masashi Tsuchiya, Kozo Takahashi, Kiyotaka Takishita
  • Journal Title: Marine Micropaleontology Volume: Vol.81 Pages: 32-42
  • DOI: doi:10.1016/j.marmicro.2011.06.007
  • Peer Reviewed
• [Journal Article] Cyanobacterial endosymbionts in the benthic dinoflagellate Sinophysis canaliculata (Dinophyceae, Dinophysiales) (2011)
  • Author(s): Laura Escalera, Beatriz Reguera, Kiyotaka Takishita, Sadaaki Yoshimatsu, Kanae Koike, Kazuhiko Koike
  • Journal Title: Protist Volume: Vol.162 Pages: 304-314
  • DOI: doi:10.1016/j.protis.2010.07.003
  • Peer Reviewed
• [Journal Article] Green-colored plastids in the dinoflagellate genus Lepidodinium are of core chlorophyte origin (2011)
  • Author(s): Takuya Matsumoto, Fumihiko Shinozaki, Tomoko Chikuni, Akinori Yabuki, Kiyotaka Takishita, Masanobu Kawachi, Takeshi Nakayama, Isao Inouye, Tetsuo Hashimoto, Yuji Inagaki
  • Journal Title: Protist Volume: Vol.162 Pages: 268-276
  • DOI: doi:10.1016/j.protis.2010.07.001
  • Peer Reviewed
• [Presentation] A novel alveolate in gill epithelium cells of bivalves with chemosynthetic bacteria inhabiting deep-sea methane seeps in Sagami Bay, Japan (2012)
  • Author(s): Fumiya Noguchi, Masaru Kawato, Takao Yoshida, Yoshihiro Fujiwara, Katsunori Fujikura, Kiyotaka Takishita
  • Organizer: Protist2012
  • Place of Presentation: Oslo University, Norway
  • Year and Date: 20120729-0803
• [Presentation] Fine structure of Telonema subtile Griessmann, 1913: A unique cytoskeletal structure among eukaryotes (2012)
  • Author(s): Akinori Yabuki, Wenche Eikrem, Kiyotaka Takishita
  • Organizer: Protist2012
  • Place of Presentation: Oslo University, Norway
  • Year and Date: 20120729-0803
• [Presentation] 放散虫と有孔虫を姉妹群とする「レタリア仮説」に基づいたアクチン,チューブリンおよびペプチド伸長因子の複雑な進化シナリオ (2012)
  • Author(s): 石谷佳之,石川奏太,神川龍馬,矢吹彬憲,土屋正史,稲垣祐司,瀧下清貴
  • Organizer: 日本地球惑星科学連合2012年大会
  • Place of Presentation: 幕張メッセ
  • Year and Date: 2012-05-24
• [Presentation] 相模湾メタン湧出活域に生息するヘイトウシンカイヒバリガイから検出された原生生物 (2011)
  • Author(s): 野口文哉,河戸勝,吉田尊雄,藤原義弘,藤倉克則,瀧下清貴
  • Organizer: 日本進化原生生物学研究会
  • Place of Presentation: 富山大学
  • Year and Date: 2011-06-11
• [Presentation] Evolutionary analysis of mitochondrial ancestry of diplomonads: Phylogenomoics of mitochondrial-related organelles in Carpediemonas-like organism (2010)
  • Author(s): Martin Kolisko, Ryoma Kamikawa, Kiyotaka Takishita, Ivan Cepicka, Qianqian Zhang, Tetsuo Hashimoto, Akinori Yabuki, Yuji Inagaki, Andrew J. Roger, Alastair G.B. Simpson
  • Organizer: Protist2012
  • Place of Presentation: Oslo University, Norway
  • Year and Date: 20100729-0803