Elucidation of the protonation mechanisms of ferredoxin-dependent bilin reductase by neutron crystallography

2011 Fiscal Year Final Research Report

• Project/Area Number: 22770096
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Structural biochemistry
• Research Institution: Ibaraki University
• Principal Investigator: UNNO Masaki 茨城大学, フロンティア応用原子科学研究センター, 准教授 (10359549)
• Research Collaborator: FUKUYAMA Keiichi 大阪大学, 理学研究科, 教授 (80032283)
• Project Period (FY): 2010 – 2011
• Keywords: 酵素

Research Abstract

We succeeded obtaining a big crystal of PcyA, a ferredoxin-dependent bilin reductase, which biosynthesizes an important pigment pytochromobilin. The crystal diffracted neutron up to 2.6 A resolution. If we can obtain bigger crystal than this one, we will able to structural analysis beyond 2 A resolution, and determine the position of hydrogen atoms in the structure. The reaction mechanism will be elucidated, soon.

Research Products

• [Journal Article] 金属タンパク質のX線結晶構造解析:ヘム分解酵素の反応中間体 (2011)
  • Author(s): 海野昌喜、松井敏高、齋藤正男
  • Journal Title: 日本結晶学会誌 Volume: 53 Pages: 213-218
  • Peer Reviewed
• [Journal Article] Enzymatic Ring-Opening Mechanism of Verdoheme by the Heme Oxygenase : A Combined X-ray Crystallography and QM/MM Study. (2010)
  • Author(s): Lai, W., Chen, H., Matsui, T., Omori, K., Unno, M., Ikeda-Saito, M., Shaik, S.
  • Journal Title: J. Am. Chem. Soc. Volume: 132 Pages: 12960-12970
  • Peer Reviewed
• [Journal Article] Solution 1H NMR characterization of substrate-free C. diphtheriae heme oxygenase : Pertinence for determining magnetic axes in paramagnetic substrate complexes. (2010)
  • Author(s): Du, Z., Unno, M., Matsui, T., Ikeda-Saito, M., La Mar, G., N.
  • Journal Title: J. Inorg. Biochem Volume: 104 Pages: 1063-1070
  • Peer Reviewed
• [Journal Article] Dioxygen Activation for the Self-Degradation of Heme : Reaction Mechanism and Regulation of Heme Oxygenase (2010)
  • Author(s): Matsui, T., Iwasaki, M., Sugiyama, R., Unno, M., Ikeda-Saito, M.
  • Journal Title: Inorg. Chem Volume: 49 Pages: 3602-3609
  • DOI: DOI:10.1021/ic901869t
  • Peer Reviewed
• [Presentation] フェレドキシン依存性ビリン還元酵素PcyAの大型結晶作製の試みと高分解能結晶構造解析 (2011)
  • Author(s): 石川久美子,伊中浩治,萩原義徳,和田啓,黒木良太,玉田太郎,福山恵一,海野昌喜
  • Organizer: 日本化学会関東支部、茨城地区交流会
  • Place of Presentation: 東海村
  • Year and Date: 2011-11-04
• [Presentation] 中性子結晶構造解析を目指したフェレドキシン依存性ビリン還元酵素PcyAの結晶成長 (2010)
  • Author(s): 石川久美子,萩原義徳,和田啓,小原裕二,黒木良太,玉田太郎,福山恵一,海野昌喜
  • Organizer: 日本結晶学会2010,創立60周年記念大会
  • Place of Presentation: 大阪
  • Year and Date: 2010-12-05
• [Presentation] 中性子結晶解析で見るビリン還元酵素のプロトンを使った反応機構 (2010)
  • Author(s): 海野昌喜
  • Organizer: 茨城県中性子利用促進研究会(生命物質構造研究会)合同研究会
  • Place of Presentation: 東京
  • Year and Date: 2010-10-01
• [Presentation] Ring Opening Mechanism of Verdoheme Catalyzed by Heme Oxygenase (2010)
  • Author(s): Toshitaka Matsui, Masaki Unno, Masao Ikeda-Saito
  • Organizer: 2010年日本生物物理学会第48回年会
  • Place of Presentation: 仙台
  • Year and Date: 2010-09-22
• [Presentation] Crystal Growth of Pcy A, a Ferredoxin-Dependent Billin Reductase, for Neutron Crystallographic Analysis (2010)
  • Author(s): Kumiko Ishikawa, Yoshinori Hagiwara, Kei Wada, Yuji Obara, Ryota Kuroki, Taro Tamada, Keiichi Fukuyama, Masaki Unno
  • Organizer: 2010年日本生物物理学会第48回年会
  • Place of Presentation: 仙台
  • Year and Date: 2010-09-22
• [Remarks]
  • URL: http://www.fas.ibaraki.ac.jp/?page_id=827