2011 Fiscal Year Final Research Report
Structural basis for specific cleavage of Lys63-linked polyubiquitin chains by Trabid
| Project/Area Number |
22770099
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Structural biochemistry
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| Research Institution | The University of Tokyo |
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Principal Investigator |
SATO Yusuke 東京大学, 放射光連携研究機構, 助教 (50568061)
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| Project Period (FY) |
2010 – 2011
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| Keywords | X線結晶解析 |
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| Research Abstract |
We prepared the mouse, zebrafish and fly Trabid OTU domain and screened against 500 crystallization conditions. However, the OTU domains of Trabid were not crystallized. On the other hand, we determined the crystal structure of the HOIL-1L NZF domain in complex with linear di-ubiquitin. Together with structure based mutagenesis experiments our structure reveals the mechanism for specific recognition of linear chains by the HOL-1L NZF domain. This result was published in PNAS
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Research Products
(3 results)
