2011 Fiscal Year Final Research Report
Structural analysis for the urea transporter
| Project/Area Number |
22770100
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Structural biochemistry
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2011
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| Keywords | X線結晶解析 |
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| Research Abstract |
We succeeded the expression and purification of the urea transporter from Desulfovibrio vulgaris. After the optimization of the detergent, we obtained the crystals using the decyl maltoside. We collected the low resolution diffraction dataset on this crystal. We expressed the mouse urea transporter as the GFP fused form. FSEC analysis revealed that the mouse urea transporter exists as the monodisperse state, suitable for the structural analysis.
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