2011 Fiscal Year Final Research Report
Dynamic structural analysis of histone in nucleosome using solid-state NMR
| Project/Area Number |
22770108
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Structural biochemistry
|
|---|
| Research Institution | Yokohama City University |
|---|
Principal Investigator |
TODOKORO Yasuto 横浜市立大学, 生命ナノシステム科学研究科, 特任助教 (00533150)
|
|---|
| Project Period (FY) |
2010 – 2011
|
| Keywords | 核磁気共鳴法 / 固体NMR / 構造生物学 / ヌクレオソーム / ヒストン / クロマチン / 天然変性 |
|---|
| Research Abstract |
In eukaryotic cells the fundamental structural unit of chromatin is a nucleosome core, consisting of two copies of four histones and DNA, whose crystalline structures have been determined. However in the crystals the N-terminal histone tails, which are responsible for gene regulations by their chemical modifications could not be well identified. Here, we have examined the structures of N-terminal histone tails by solid-state NMR and suggested that the histone tails hold flexible random coil structures, which might play significant roles in gene regulations.
|
