High pressure NMR study for conformational fluctuation of polyubiquitin

2011 Fiscal Year Final Research Report

• Project/Area Number: 22770114
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Structural biochemistry
• Research Institution: Ritsumeikan University
• Principal Investigator: KITAHARA Ryo 立命館大学, 薬学部, 准教授 (70512284)
• Project Period (FY): 2010 – 2011
• Keywords: 蛋白質 / 圧力 / NMR

Research Abstract

We investigated conformational fluctuation of Lys48-linked linear diubiquitin, as a minimum model of polyubiquitin chains, by using high-pressure NMR spectroscopy. Comparing pressure induced changes in chemical shifts between mono-ubiquitin and diubiquitin, we found that pressure stabilizes the "open" orientation of the two subunits for diubiquitin and the subunits have similar intrinsic motion to those of mono-ubiquitin. It is suggested that the intrinsic fluctuation of the subunits plays an essential role in the adaptation of global conformation of diubiquitin to the environment.

Research Products

• [Journal Article] A delicate interplay of structure, dynamics and thermodynamics for function. A high pressure NMR and fluorescence study of Outer surface protein A. (2012)
  • Author(s): Kitahara R., Simorellis A. K., Hata K., Maeno A., Yokoyama, S., Koide S. and Akasaka K.
  • Journal Title: Biophysical Journal Volume: 102 Pages: 916-926
  • Peer Reviewed
• [Journal Article] Structural plasticity of staphylococcal nuclease probed by perturbation with pressure and pH (2011)
  • Author(s): Ryo Kitahara, Kazumi Hata, Akihiro Maeno and Kazuyuki Akasaka, Michael Chimenti, Bertrand Garcia-Moreno, E. Martin A. Schroer, Christoph Jeworrek, Metin Tolan, Roland Winter, Julien Roche, Christian Roumestand, Karine Montet de Guillen and Catherine A. Ro
  • Journal Title: Proteins Volume: 79 Pages: 1293-1305
  • Peer Reviewed
• [Presentation] 準安定構造に着目した構造-機能相関:ユビキチン、ジユビキチン (2012)
  • Author(s): 北原亮
  • Organizer: 第3回バイオ単分子研究会(招待講演)
  • Place of Presentation: 新世代研究所会議室(東京都)
  • Year and Date: 2012-03-09
• [Presentation] ESIGN STRATEGY FOR HIGH FREE-ENERGY STATES OF PROTEINS BASED ON HIGH-PRESSURE NMR STUDY : ALTERNATIVELY FOLDED CONFORMATION OF UBIQUITIN (2012)
  • Author(s): Kitahara R., Kitazawa S., Yagi-Utsumi M., Ido A., Urushitani M., Sugase K., Kato K.
  • Organizer: Biophysical Society 56th Annual Meeting
  • Place of Presentation: サンディエゴUSA
  • Year and Date: 2012-02-26
• [Presentation] 高圧力NMRによる構造生物学の新展開 (2011)
  • Author(s): 北原亮
  • Organizer: NEDO特別講演会(招待講演)
  • Place of Presentation: 東京大学薬学部講堂(東京都)
  • Year and Date: 2011-10-13
• [Presentation] 蛋白質の準安定状態に着目した新しい構造生物学の展開 (2011)
  • Author(s): 北原亮
  • Organizer: 蛋白質研究所セミナー(招待講演)
  • Place of Presentation: 大阪大学蛋白研究所(大阪府)
  • Year and Date: 2011-07-29
• [Presentation] タンパク質の高エネルギー構造に注目した新しい構造生物学の展開 (2011)
  • Author(s): 北原亮
  • Organizer: 第2回若手育成講習会(招待講演)
  • Place of Presentation: 大阪大学蛋白研究所(大阪府)
  • Year and Date: 2011-04-26
• [Book] 広がるNMRの世界 (2011)
  • Author(s): 朝倉哲郎
  • Total Pages: 70-73
  • Publisher: コロナ社
• [Remarks]
  • URL: http://www.ritsumei.ac.jp/pharmacy/kitahara/kitahara_lab.html