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2011 Fiscal Year Final Research Report

High pressure NMR study for conformational fluctuation of polyubiquitin

Research Project

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Project/Area Number 22770114
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Structural biochemistry
Research InstitutionRitsumeikan University

Principal Investigator

KITAHARA Ryo  立命館大学, 薬学部, 准教授 (70512284)

Project Period (FY) 2010 – 2011
Keywords蛋白質 / 圧力 / NMR
Research Abstract

We investigated conformational fluctuation of Lys48-linked linear diubiquitin, as a minimum model of polyubiquitin chains, by using high-pressure NMR spectroscopy. Comparing pressure induced changes in chemical shifts between mono-ubiquitin and diubiquitin, we found that pressure stabilizes the "open" orientation of the two subunits for diubiquitin and the subunits have similar intrinsic motion to those of mono-ubiquitin. It is suggested that the intrinsic fluctuation of the subunits plays an essential role in the adaptation of global conformation of diubiquitin to the environment.

  • Research Products

    (9 results)

All 2012 2011 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (5 results) Book (1 results) Remarks (1 results)

  • [Journal Article] A delicate interplay of structure, dynamics and thermodynamics for function. A high pressure NMR and fluorescence study of Outer surface protein A.2012

    • Author(s)
      Kitahara R., Simorellis A. K., Hata K., Maeno A., Yokoyama, S., Koide S. and Akasaka K.
    • Journal Title

      Biophysical Journal

      Volume: 102 Pages: 916-926

    • Peer Reviewed
  • [Journal Article] Structural plasticity of staphylococcal nuclease probed by perturbation with pressure and pH2011

    • Author(s)
      Ryo Kitahara, Kazumi Hata, Akihiro Maeno and Kazuyuki Akasaka, Michael Chimenti, Bertrand Garcia-Moreno, E. Martin A. Schroer, Christoph Jeworrek, Metin Tolan, Roland Winter, Julien Roche, Christian Roumestand, Karine Montet de Guillen and Catherine A. Ro
    • Journal Title

      Proteins

      Volume: 79 Pages: 1293-1305

    • Peer Reviewed
  • [Presentation] 準安定構造に着目した構造-機能相関:ユビキチン、ジユビキチン2012

    • Author(s)
      北原亮
    • Organizer
      第3回バイオ単分子研究会(招待講演)
    • Place of Presentation
      新世代研究所会議室(東京都)
    • Year and Date
      2012-03-09
  • [Presentation] ESIGN STRATEGY FOR HIGH FREE-ENERGY STATES OF PROTEINS BASED ON HIGH-PRESSURE NMR STUDY : ALTERNATIVELY FOLDED CONFORMATION OF UBIQUITIN2012

    • Author(s)
      Kitahara R., Kitazawa S., Yagi-Utsumi M., Ido A., Urushitani M., Sugase K., Kato K.
    • Organizer
      Biophysical Society 56th Annual Meeting
    • Place of Presentation
      サンディエゴUSA
    • Year and Date
      2012-02-26
  • [Presentation] 高圧力NMRによる構造生物学の新展開2011

    • Author(s)
      北原亮
    • Organizer
      NEDO特別講演会(招待講演)
    • Place of Presentation
      東京大学薬学部講堂(東京都)
    • Year and Date
      2011-10-13
  • [Presentation] 蛋白質の準安定状態に着目した新しい構造生物学の展開2011

    • Author(s)
      北原亮
    • Organizer
      蛋白質研究所セミナー(招待講演)
    • Place of Presentation
      大阪大学蛋白研究所(大阪府)
    • Year and Date
      2011-07-29
  • [Presentation] タンパク質の高エネルギー構造に注目した新しい構造生物学の展開2011

    • Author(s)
      北原亮
    • Organizer
      第2回若手育成講習会(招待講演)
    • Place of Presentation
      大阪大学蛋白研究所(大阪府)
    • Year and Date
      2011-04-26
  • [Book] 広がるNMRの世界2011

    • Author(s)
      朝倉哲郎
    • Total Pages
      70-73
    • Publisher
      コロナ社
  • [Remarks]

    • URL

      http://www.ritsumei.ac.jp/pharmacy/kitahara/kitahara_lab.html

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Published: 2013-07-31  

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