2011 Fiscal Year Final Research Report
High pressure NMR study for conformational fluctuation of polyubiquitin
Project/Area Number |
22770114
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Single-year Grants |
Research Field |
Structural biochemistry
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Research Institution | Ritsumeikan University |
Principal Investigator |
KITAHARA Ryo 立命館大学, 薬学部, 准教授 (70512284)
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Project Period (FY) |
2010 – 2011
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Keywords | 蛋白質 / 圧力 / NMR |
Research Abstract |
We investigated conformational fluctuation of Lys48-linked linear diubiquitin, as a minimum model of polyubiquitin chains, by using high-pressure NMR spectroscopy. Comparing pressure induced changes in chemical shifts between mono-ubiquitin and diubiquitin, we found that pressure stabilizes the "open" orientation of the two subunits for diubiquitin and the subunits have similar intrinsic motion to those of mono-ubiquitin. It is suggested that the intrinsic fluctuation of the subunits plays an essential role in the adaptation of global conformation of diubiquitin to the environment.
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Research Products
(9 results)
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[Journal Article] Structural plasticity of staphylococcal nuclease probed by perturbation with pressure and pH2011
Author(s)
Ryo Kitahara, Kazumi Hata, Akihiro Maeno and Kazuyuki Akasaka, Michael Chimenti, Bertrand Garcia-Moreno, E. Martin A. Schroer, Christoph Jeworrek, Metin Tolan, Roland Winter, Julien Roche, Christian Roumestand, Karine Montet de Guillen and Catherine A. Ro
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Journal Title
Proteins
Volume: 79
Pages: 1293-1305
Peer Reviewed
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