2012 Fiscal Year Final Research Report
pproach for circadian oscillatory mechanisms based on phosphorylation rhythms of clock proteins
| Project/Area Number |
22770122
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Functional biochemistry
|
|---|
| Research Institution | The University of Tokyo |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2010 – 2012
|
| Keywords | 脳・神経 / 動物行動 / サーカディアンリズム / 概日時計 / 時計タンパク質 / リン酸化シグナリング / ChIP-Seq / 次世代シーケンサー |
|---|
| Research Abstract |
In the mammalian circadian clockwork, CLOCK and BMAL1 bind to DNA and play an important role in the transcriptional feedback loops. In this study, we performed ChIP-Seq analysis and determined all the CLOCK-binding regions in genome-wide manner. RNA-Seq analysis showed circadian profiles of all the transcripts. Furthermore, we identified JNK as novel protein kinase for phosphorylation of CLOCK-BMAL1 complex, and behavioral analysis of JNK3-KO mice showed that JNK is a key player in the photic regulation of circadian clockwork.
|
