2011 Fiscal Year Final Research Report
Characterization of the biosynthesis system for thiouridine in tRNA
| Project/Area Number |
22770140
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Functional biochemistry
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| Research Institution | National Institute of Advanced Industrial Science and Technology |
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Principal Investigator |
SHIGI Naoki 独立行政法人産業技術総合研究所, バイオメディシナル情報研究センター, 研究員 (20392623)
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| Project Period (FY) |
2010 – 2011
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| Keywords | 核酸 / タンパク質 / 酵素 / 微生物 |
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| Research Abstract |
The biosynthesis pathway of thiouridine found in tRNA of thermophilic bacteria was analyzed. An in vitro reconstitution system for tRNA thiolation reaction was constructed. By using an in vivo system, a reaction mechanism for the thiolation enzyme TtuA was suggested. It was suggested that one of the biosynthesis protein, TtuB, also function as a protein modifier, which regulates the thiolation reaction of tRNA. This suggests an ancient origin of the eukaryotic ubiquitin system.
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