2011 Fiscal Year Final Research Report
Time resolved infrared spectroscopy of cytochrome c oxidase in aqueous solution
| Project/Area Number |
22770154
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biophysics
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| Research Institution | Okayama University of Science (2011)
University of Hyogo (2010) |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2011
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| Keywords | 膜蛋白質 / 生体エネルギー / 赤外分光 / プロトンポンプ |
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| Research Abstract |
The newly developed time-resolved infrared system was constructed using fs infrared laser for a light source to investigate a proton pumping mechanism of cytochrome c oxidase(C_cO). The function of the O_2 reduction site using CO as an analogue of O_2 was investigated by this new system which is capable of analyzing C_cO in H_2O solution. The results indicate the existence of a conformational relay system which plays a key role in connecting the CuB site and the water channel for efficient collection of protons being pumped. It was found that transient CO-binding to Cu_B, after Fe_<a3>-CO photolysis, opens the water-channel.
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