The identification and molecular function of nuclear glycosyl transferase complex

2011 Fiscal Year Final Research Report

• Project/Area Number: 22770165
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Molecular biology
• Research Institution: The University of Tokyo
• Principal Investigator: FUJIKI Ryoji 東京大学, 分子細胞生物学研究所, 助教 (40534516)
• Project Period (FY): 2010 – 2011
• Keywords: 転写制御 / クロマチン / 糖修飾

Research Abstract

In this research, we focused on the molecular function of O-linked N-acetylglucosamine transferase(OGT), which was identified as a co-activator of nuclear receptor(NR)-mediated transcription in our previous study. To understand the molecular basis for OGT to activate transcription from chromatin, we purified its interacting partners by affinity purification, and identified several chromatin regulatory factors including histone methyltransferases. In addition, we found that histone was a novel substrate for OGT. Together, this modification might be a substantial epigenetic code for the regulation of eukaryotic gene expression network.

Research Products

• [Journal Article] GlcNAcylation of histone H2B facilitates its monoubiquitination (2011)
  • Author(s): Fujiki R., Hashiba W., Sekine H., Yokoyama A., Chikanishi T., Ito S., Imai Y., Kim J., He HH., Igarashi K., Kanno J., Ohtake F., Kitagawa H., Roeder RG., Brown M., Kato S.
  • Journal Title: Nature Volume: 480 Pages: 557-560
  • Peer Reviewed
• [Journal Article] Regulated histone methyltransferase and demethylase complexes in the control of genes by nuclear receptor (2011)
  • Author(s): Yokoyama A., Fujiki R., Ohtake F., Kato S.
  • Journal Title: Cold Spring Harb SympQuant Biol
  • Peer Reviewed
• [Journal Article] Multiple post-translational modifications in hepatocyte nuclear factor 4alpha (2011)
  • Author(s): Yokoyama A., Katsura S., Ito R., Hashiba W., Sekine H., Fujiki R., Kato S.
  • Journal Title: Biochem Biophys Res Commun Volume: 410 Pages: 749-753
  • Peer Reviewed
• [Journal Article] Transcriptional controls by nuclear fat-soluble vitamin receptors through chromatin reorganization (2011)
  • Author(s): Kato S., Fujiki R.
  • Journal Title: Biosci Biotechnol Biochem Volume: 75 Pages: 410-413
  • Peer Reviewed
• [Journal Article] Nuclear receptor coregulators merge transcriptional coregulation with epigenetic regulation (2011)
  • Author(s): Kato S., Yokoyama A., Fujiki R.
  • Journal Title: Trends Biochem Sci Volume: 36 Pages: 272-281
  • Peer Reviewed
• [Journal Article] Williams syndrome is an epigenome-regulator disease (2011)
  • Author(s): Kitagawa H., Fujiki R., Yoshimura K., Oya H., Kato S.
  • Journal Title: Endocr J Volume: 58 Pages: 77-85
  • Peer Reviewed
• [Journal Article] KIAA1718 is a histone demethylase that erases repressive histone methyl marks (2010)
  • Author(s): Yokoyama A, Okuno Y, Chikanishi T, Hashiba W, Sekine H. Fujiki R. Kato S.
  • Journal Title: Genes Cells Volume: 15 Pages: 867-873
  • Peer Reviewed
• [Journal Article] Double PHD fingers protein DPF2 recognizes acetylated histones and suppress the function of estrogen-related receptor alpha through histone deacetylase 1 (2010)
  • Author(s): Matsuyama R, Takada I, Yokoyama A, Fujiyama-Nakamura S, Tsuji N, Kitagawa H, Fujiki R., Kim M, Kouzu-Fujita M, Yano T, Kato S.
  • Journal Title: J Biol Chem Volume: 285 Pages: 18166-18176
  • Peer Reviewed
• [Journal Article] Glucose-induced expression of MIP1-genes requires O-GlcNAc transferase in monocytes (2010)
  • Author(s): Chikanishi T., Fujiki R., Hashiba W., Sekine H., Yokoyama A., Kato S.
  • Journal Title: Biochem Biophys Res Commun Volume: 394 Pages: 865-870
  • Peer Reviewed
• [Journal Article] Hormonal gene regulation through DNA methylation and demethylation (2010)
  • Author(s): Kouzmenko A., Ohtake F., Fujiki R., Kato S.
  • Journal Title: Epigenomics Volume: 2 Pages: 765-774
  • Peer Reviewed
• [Journal Article] Role of nuclear O-Glycosylation in epigenetic regulation (2010)
  • Author(s): Fujiki R., Chikanishi T., Hashiba W., Kato S.
  • Journal Title: Tanpakushitsu Kakusan Koso(Japanese) Volume: 55 Pages: 61-68
• [Journal Article] A histone chaperone, DEK, transcriptionally coactivates a nuclear receptor (2010)
  • Author(s): Sawatsubashi S., Murata T., Lim J., Fujiki R., Ito S., Suzuki E., Tanabe M., Zhao Y., Kimura S., Fujiyama S., Ueda T., Umetsu D., Ito T., Takeyama K., Kato S.
  • Journal Title: Genes Dev Volume: 24 Pages: 159-170
  • Peer Reviewed
• [Presentation] Roeder, Shigeaki Kato, GlcNActylation of a histone methyltransferase in regtinoic acid-induced granulopoiesis (2011)
  • Author(s): Ryoji Fujiki, Toshihiro Chikanishi, Waka Hashiba, Robert G.
  • Organizer: Keystone Symposia : Histone Code : Fact or Fiction?
  • Place of Presentation: Utah, USA
  • Year and Date: 20110110-15
• [Presentation] 核内O-GlcNAc修飾を介するエピゲノム制御機構の新たな作用点の探索 (2010)
  • Author(s): 藤木亮次,近西俊洋,橋場和華,関根弘樹,横山敦,加藤茂明
  • Organizer: 第33回日本分子生物学会年会,第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 20101207-10
• [Presentation] 核内糖修飾を介するエピジェネティクスの制御機構 (2010)
  • Author(s): 藤木亮次,加藤茂明
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都
  • Year and Date: 20100921-24
• [Patent(Industrial Property Rights)] ヒストンタンパク質のグリコシル化を検出するための方法、並びに当該方法に用いられる抗体 (2010)
  • Inventor(s): 加藤茂明、藤木亮次
  • Industrial Property Rights Holder: 東京大学
  • Industrial Property Number: 特願、2010-229477
  • Filing Date: 2010-10-12