A genetical screening of a protein trap library for active chromatin-binding factors on polytene chromosome in Drosophila

2011 Fiscal Year Final Research Report

• Project/Area Number: 22770166
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Molecular biology
• Research Institution: Gunma University
• Principal Investigator: SAWATSUBASHI Shun 群馬大学, 生体調節研究所, 助教 (70535103)
• Research Collaborator: LIM Jinseon 東京大学, 分子細胞生物学研究所, 博士課程
• Project Period (FY): 2010 – 2011
• Keywords: 転写 / クロマチン / 核内受容体 / エクダイソン

Research Abstract

It is evident that gene regulation is coupled with chromatin reorganaization, but factors and molecular basis involving in this process remain to be identified. In the present study, factors responsible for ecdysone-induced puff formation were genetically screened in Drosophila fly by establishing an EGFP protein-trap approach. Among the candidates, Drosophila CG11138/Ringfect was found as a co-activator for ecdysone receptor (EcR). Through biochemical characterization, Ringfect could be shown to serve as a histone chaperon. We describe the identification of novel puff-localized factors by screening the protein trap transgenic fly library. Drosophila CG11138/Ringfect and enolase were genetically identified as a puff-localized factor and a co-activator, and interacted with EcR in S2 cell. Thus, the present study suggests that Ringfect and enolase are categorized as a novel class of nuclear receptor co-activators.

Research Products

• [Journal Article] Epigenetic silencing of core histone genes by HERS in drosophila (2012)
  • Author(s): Ito S, Fujiyama-Nakamura S, Kimura S, Lim J, Kamoshida Y, Shiozaki-Sato Y, Sawatsubashi S, Suzuki E, Tanabe M, Ueda T, Murata T, Kato H, Ohtake F, Fujiki R, Miki T, Kouzmenko A, Takeyama K, Kato S
  • Journal Title: Molecular Cell Volume: Vol.45 (4) Pages: 494-504
  • Peer Reviewed
• [Journal Article] CDP/cut is an osteoblastic coactivator of the vitamin D receptor (VDR) (2010)
  • Author(s): Ochiai E, Kitagawa H, Takada I, Fujiyama S, Sawatsubashi S, Kim MS, Mezaki Y, Tsushima Y, Takagi K, Azuma Y, Takeyama K, Yamaoka K, Kato S, Kamimura T
  • Journal Title: J Bone Miner. Res. Volume: Vol.25 (5) Pages: 1157-1166
  • Peer Reviewed
• [Presentation] 多糸染色体から理解するクロマチン構造調節を介したゲノムデコード-新規ヒストンシャペロンDosophila DEKによるエクダイソン受容体転写機構の解明- (2012)
  • Author(s): 沢津橋俊
  • Organizer: 日本農芸化学会2012年度大会
  • Place of Presentation: 京都女子大学(京都府)
  • Year and Date: 2012-03-25
• [Presentation] DEKはヒストンシャペロンとして機能する新規転写活性化共役因子である (2011)
  • Author(s): 沢津橋俊
  • Organizer: 日本農芸化学会2011年度大会
  • Place of Presentation: 京都女子大学(京都府)
  • Year and Date: 2011-03-27
• [Presentation] A Histone chaperone DEK transcriptionally coactivating a nuclear receptor : a functional link to leukemia (2011)
  • Author(s): Sawatsubashi S
  • Organizer: Keystone Symposia (Histone Code : Fact or Fiction?)
  • Place of Presentation: ミッドウェイ(米国・ユタ州)
  • Year and Date: 2011-01-13
• [Presentation] A Histone chaperone DEK transcriptionally coactivating a nuclear receptor : a functional link to leukemia (2010)
  • Author(s): 沢津橋俊
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸ポートアイランド(兵庫県)
  • Year and Date: 2010-12-08