2011 Fiscal Year Final Research Report
The molecular mechanism of the endogenous siRNA biogenesis
| Project/Area Number |
22770174
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Molecular biology
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| Research Institution | Keio University |
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Principal Investigator |
MIYOSHI Keita 慶應義塾大学, 医学部, 助教 (20423395)
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| Project Period (FY) |
2010 – 2011
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| Keywords | RNAサイレンシング / RNA干渉 / 細胞内輸送 |
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| Research Abstract |
Small RNAs provide specificity to protein effector complexes that repress mRNA transcription or translation, or catalyze mRNA destruction. In this research program, we tried to gain a better under standing of the molecular mechanisms underlying the endogenous siRNA(esiRNA) biogenesis. Using Drosophila cell, we found a novel cytoplasmic granules which contains the esiRNA biogenesis proteins and that this granule is necessary for the specific association between esiRNA and the effector complex.
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[Journal Article] Chromatin-associated RNA interference components contribute to transcriptional regulation in Drosophila2011
Author(s)
Cernilogar, F. M., Onorati, M. C., Kothe, G. O., Burroughs, A. M., Parsi, K. M., Breiling, A., Sardo, F. L., Saxena, A., Miyoshi, K., Siomi, H., Siomi, M. C., Carninci, P., Gilmour D. S., Corona D. F. V., and Orlando, V.
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Journal Title
Nature
Volume: 480
Pages: 391-395
Peer Reviewed
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[Presentation] Specific requirement for Dicer2 and Loquacious-PD isoform in endogenous small-interfering RNA biogenesis in Drosophila. Keystone symposia2011
Author(s)
Miyoshi, K., Ogino, A., Siomi, H., Siomi, M. C.
Organizer
Mechanism and Biology of Silencing
Place of Presentation
Portola Hotel & Spa, Monterey, California, USA
Year and Date
20110320-25
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[Presentation] Roles of Dicer2 partners, R2D2 and Loqs-PD, in the esiRNA biogenesis pathway in Drosophila2011
Author(s)
Miyoshi, K., Ogino, A., Siomi, M. C., Siomi, H.
Organizer
Sixteenth annual meeting of the RNA society
Place of Presentation
Kyoto international conference hall, JAPAN
Year and Date
2011-06-17
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[Presentation] Specific requirement for Dicer2 and Loquacious-PD isoform in endogenous small-interfering RNA biogenesis in Drosophila. Keystone symposia2010
Author(s)
Miyoshi, K., Miyoshi, T., Hartig, J. V., Siomi, H., Siomi, M. C.
Organizer
RNA Silencing : Mechanism, Biology and Application
Place of Presentation
Keystone Resort, USA
Year and Date
20100114-19
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[Presentation] The recognition of different Dicer-Loqs complexes to small RNA precursors in Drosophila2010
Author(s)
Miyoshi, K., Ogino, A., Miyoshi, T., Hartig, J. V., Siomi, H., Siomi, M. C.
Organizer
Exciting Biologies : Biology of Recognition
Place of Presentation
Sentosa Resort & Spa, SINGAPORE
Year and Date
2010-10-08
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