Analysis of the mRNA silencing mechanism by small RNA in E. coli

2011 Fiscal Year Final Research Report

• Project/Area Number: 22770175
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Molecular biology
• Research Institution: Suzuka University of Medical Science
• Principal Investigator: MORITA Teppei 鈴鹿医療科学大学, 薬学部, 助手 (10444366)
• Project Period (FY): 2010 – 2011
• Keywords: 低分子RNA / 遺伝子発現抑制 / 大腸菌 / 塩基対形成 / RNAシャペロン

Research Abstract

SgrS is a well-characterized Hfq-binding sRNA. In this project, I tried to define the SgrS region required for translation repression of ptsG mRNA, and to clarify the mechanism by which Hfq promotes the base-pairing between SgrS and ptsG mRNA. The major findings are : 1) SgrS168-181(14 nt) is a minimal base-pair region for translational repression of ptsG mRNA ; 2) the polyU tail of rho-independent terminator is essential for Hfq action ; 3) the subregion between 711 and 750 of RNase E is sufficient for the functional interaction with Hfq to support the rapid degradation of ptsG mRNA.

Research Products

• [Journal Article] PolyU tail of rho-independent terminator of bacterial small RNAs is essential for Hfq action (2011)
  • Author(s): Hironori Otaka, Hirokazu Ishikawa, Teppei Morita, and Hiroji Aiba
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: 108(32) Pages: 13059-13064
  • Peer Reviewed
• [Journal Article] 原核生物の制御RNA (2011)
  • Author(s): 森田鉄兵、饗場弘二
  • Journal Title: 医学のあゆみ Volume: 238(5) Pages: 394-399
• [Journal Article] Hfq binding at RhlB-recognition region of RNase E is crucial for the rapid degradation of target mRNAs mediated by sRNAs in E (2011)
  • Author(s): Ikeda, Y., Yagi, M., Morita, T., and Aiba, H
  • Journal Title: Coli. Mol. Microbiol Volume: 79(2) Pages: 419-432
  • Peer Reviewed
• [Journal Article] RNase E action at a distance : degradation of target mRNAs mediated by an Hfq-binding small RNA in bacteria (2011)
  • Author(s): Morita, T., and Aiba, H
  • Journal Title: Genes Dev Volume: 25(4) Pages: 294-298
  • Peer Reviewed
• [Journal Article] A minimal base-pairing region of a bacterial small RNA SgrS required for translational repression of ptsG mRNA (2010)
  • Author(s): Maki, K., Morita, T., Otaka, H., and Aiba, H
  • Journal Title: Mol. Microbiol Volume: 76(3) Pages: 782-792
  • Peer Reviewed
• [Presentation] (2011)
  • Author(s): 森田鉄兵、大鷹弘紀、石川博一、饗場弘二
  • Organizer: 第5回日本ゲノム微生物学会若手の会
  • Place of Presentation: ろうきん研修所富士センター(静岡)
  • Year and Date: 20110929-30
• [Presentation] 遺伝子発現制御機構における大腸菌低分子RNA3'末端ポリU配列の役割 (2011)
  • Author(s): 大鷹弘紀、石川博一、森田鉄兵、饗場弘二
  • Organizer: RNAフロンティアミーティング
  • Place of Presentation: あいち健康プラザ(愛知)
  • Year and Date: 20110830-0901
• [Presentation] 遺伝子発現制御機構における大腸菌低分子RNA3'末端ポリU配列の役割 (2011)
  • Author(s): 大鷹弘紀、石川博一、森田鉄兵、饗場弘二
  • Organizer: 第5回細菌学若手コロッセウム
  • Place of Presentation: 桂浜荘(高知)
  • Year and Date: 20110808-10
• [Presentation] 大腸菌小分子RNAのHfq作用領域の解析:塩基対形成領域と転写終結領域間の役割 (2011)
  • Author(s): 石川博一、大鷹弘紀、森田鉄兵、饗場弘二
  • Organizer: 第5回細菌学若手コロッセウム
  • Place of Presentation: 桂浜荘(高知)
  • Year and Date: 20110808-10
• [Presentation] Poly tail of rho-independent terminator of bacterial small RNA acts an essential Hfq-binding site for riboregulation. (2011)
  • Author(s): 大鷹弘紀、石川博一、森田鉄兵、饗場弘二
  • Organizer: The 16^<th> Annual meeting of the RNA society,第13回日本RNA学会総会合同会
  • Place of Presentation: 京都国際会議場(京都)
  • Year and Date: 20110614-18
• [Presentation] Identification of Hfq binding sites on RNase E in E. coli (2011)
  • Author(s): 池田幸樹、八木美江子、森田鉄兵、金井昭夫、饗場弘二
  • Organizer: The 16^<th> Annual meeting of the RNA society,第13回日本RNA学会総会合同会
  • Place of Presentation: 京都国際会議場(京都)
  • Year and Date: 20110614-18
• [Presentation] Both polyU tail and the upstream region of rho-independent terminator are required for the functional interaction of SgrS with Hfq (2011)
  • Author(s): 石川博一、大鷹弘紀、森田鉄兵、饗場弘二
  • Organizer: The 16^<th> Annual meeting of the RNA society,第13回日本RNA学会総会合同会
  • Place of Presentation: 京都国際会議場(京都)
  • Year and Date: 20110614-18
• [Presentation] Generation of functional E. coli DicF small RNA and the role of the 3' polyU stretch in Hfq binding (2011)
  • Author(s): 森田鉄兵、飯田芳文、饗場弘二
  • Organizer: The 16^<th> Annual meeting of the RNA society,第13回日本RNA学会総会合同会
  • Place of Presentation: 京都国際会議場(京都)
  • Year and Date: 20110614-18
• [Presentation] 遺伝子発現制御機構における大腸菌低分子RNA3'末端ポリU配列の役割 (2011)
  • Author(s): 大鷹弘紀、石川博一、森田鉄兵、饗場弘二
  • Organizer: 第8回21世紀大腸菌研究会
  • Place of Presentation: ホテル木曽路(長野)
  • Year and Date: 20110518-19
• [Presentation] 大腸菌小分子RNAのHfq作用領域の解析:塩基対形成領域と転写終結領域間の役割 (2011)
  • Author(s): 石川博一、大鷹弘紀、森田鉄兵、饗場弘二
  • Organizer: 第8回21世紀大腸菌研究会
  • Place of Presentation: ホテル木曽路(長野)
  • Year and Date: 20110518-19
• [Presentation] Identification of Hfq binding sites on RNase E in E. coli (2010)
  • Author(s): 池田幸樹、八木美江子、森田鉄兵、饗場弘二
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 兵庫県神戸市
  • Year and Date: 20101207-10
• [Presentation] 大腸菌小分子RNAであるSgrSの機能解析 (2010)
  • Author(s): 森田鉄兵、牧貴美香、大鷹弘紀、石川博一、饗場弘二
  • Organizer: 第4回日本ゲノム微生物学会若手の会
  • Place of Presentation: 兵庫県神戸市
  • Year and Date: 20101001-02
• [Presentation] 大腸菌小分子RNAであるSgrSの機能解析 (2010)
  • Author(s): 森田鉄兵、牧貴美香、大鷹弘紀、石川博一、饗場弘二
  • Organizer: 第4回細菌学・若手コロッセウム
  • Place of Presentation: 静岡県伊豆市
  • Year and Date: 20100826-28
• [Presentation] Heterogeneity of RNA degradosome of Escherichia coli : RNase E forms several distinct complexes with partner proteins (2010)
  • Author(s): 池田幸樹、八木美江子、森田鉄兵、饗場弘二
  • Organizer: International symposium on Biodiversity Sciences 2010
  • Place of Presentation: 愛知県名古屋市
  • Year and Date: 20100731-0803
• [Presentation] Implication of spacer length between base-pairing and Hfq-binding region in bacterial small RNA function (2010)
  • Author(s): 石川博一、大鷹弘紀、森田鉄兵、饗場弘二
  • Organizer: International symposium on Biodiversity Sciences 2010
  • Place of Presentation: 愛知県名古屋市
  • Year and Date: 20100731-0803
• [Presentation] The 3' terminator poly-U tract of SgrS plays a crucial role in Hfq-dependent gene silencing (2010)
  • Author(s): 大鷹弘紀、石川博一、森田鉄兵、饗場弘二
  • Organizer: International symposium on Biodiversity Sciences 2010
  • Place of Presentation: 愛知県名古屋市
  • Year and Date: 20100731-0803
• [Presentation] SgrS3'末端のポリU配列はRNA抑制機能に重要な役割を果たす (2010)
  • Author(s): 大鷹弘紀、石川博一、森田鉄兵、饗場弘二
  • Organizer: 第12回日本RNA学会年会
  • Place of Presentation: 東京都千代田区
  • Year and Date: 20100727-29
• [Presentation] 大腸菌小分子RNAの作用機構の解析:塩基対形成領域とHfq結合領域間の距離の重要性 (2010)
  • Author(s): 石川博一、大鷹弘紀、森田鉄兵、饗場弘二
  • Organizer: 第12回日本RNA学会年会
  • Place of Presentation: 東京都千代田区
  • Year and Date: 20100727-29
• [Presentation] Identification of Hfq binding sites onRNase E in E. coli (2010)
  • Author(s): 池田幸樹、八木美江子、森田鉄兵、饗場弘二
  • Organizer: The 11^<th> Asian and Oceanian Conference on Transcription
  • Place of Presentation: 沖縄県国頭郡
  • Year and Date: 20100701-05
• [Presentation] Implication of spacer length between base-pairing and Hfq-binding region in bacterial small RNA function (2010)
  • Author(s): 石川博一、大鷹弘紀、森田鉄兵、饗場弘二
  • Organizer: The 11^<th> Asian and Oceanian Conference on Transcription
  • Place of Presentation: 沖縄県国頭郡
  • Year and Date: 20100701-05
• [Presentation] 大腸菌RNase E/Hfqの結合領域の同定 (2010)
  • Author(s): 池田幸樹、八木美江子、森田鉄兵、饗場弘二
  • Organizer: 第7回21世紀大腸菌研究会
  • Place of Presentation: 熊本県阿蘇郡
  • Year and Date: 20100603-04
• [Presentation] 大腸菌sRNAであるSgrSの機能領域の解析 (2010)
  • Author(s): 石川博一、大鷹弘紀、森田鉄兵、饗場弘二
  • Organizer: 第7回21世紀大腸菌研究会
  • Place of Presentation: 熊本県阿蘇郡
  • Year and Date: 20100603-04